半岛bd体育手机客户端 介绍
目录号
Serial |
GNM21 |
标识符
Identifier |
CSTR:19375.09.3101MOUGNM21 |
描述
Description |
这株细胞的细胞骨架染色突出呈现肌动蛋白，在细胞质中有丰富的平行微丝。 有报道称在1uM血管紧张素II存在下，它们会收缩。 细胞在软琼脂中形成克隆，SV40大T抗原阳性。
在本库通过支原体检测。 |
动物种别
Organism |
小鼠 |
性别
Gender |
*** |
组织来源
Tissue and Cell Type |
肾小球系膜细胞 |
形态
Morphology |
心肌成纤维细胞样 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，71.25%；F-12培养基(GIBCO,货号21700075，添加L-glutamine 150mg/l , NaHCO3 1.5g/L)，23.75%；添加14mM HEPES；优质胎牛血清，5%-10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
22754: MacKay K, et al. Glomerular epithelial, mesangial, and endothelial cell lines from transgenic mice.Kidney Int. 33: 677-684, 1988. PubMed: 283553951029: Robey RB, et al. Regulation of mesangial cell hexokinase activity by PKC and the classic MAPKpathway. Am. J. Physiol. 277: F742-F749, 1999. PubMed: 1056423751030: Robey RB, et al. Thrombin is a novel regulator of hexokinase activity in mesangial cells. Kidney Int.57: 2308-2318, 2000. PubMed: 1084460161211: Robey RB, et al. Regulation of mesangial cell hexokinase activity and expression by heparin-bindingepidermal growth factor-like growth factor: epidermal growth factors and phorbol esters increase glucosemetabolism via a common mechanism involving classic mitogen-activated protein kinase pathway activationand induction of hexokinase II expression. J. Biol. Chem. 277: 14370-14378, 2002. PubMed: 1178248661224: Maile S, et al. The morphology of mesangial cells cultured at high density and in collagen gels. Histol.Histopathol. 15: 403-414, 2000. PubMed: 1080935861225: Coy PE, et al. LPA is a novel lipid regulator of mesangial cell hexokinase activity and HKII isoformexpression. Am. J. Physiol. Renal Physiol. 283: F271-F279, 2002. PubMed: 1211051090657: Taneja N, et al. Proinflammatory interleukin-1 cytokines increase mesangial cell hexokinase activityand hexokinase II isoform abundance. Am. J. Physiol. Cell Physiol. 287: C548-C557, 2004. PubMed:15070811 |
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