半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-5036 |
标识符
Identifier |
CSTR:19375.09.3101MOUSCSP5036 |
描述
Description |
该细胞株建自Abelson小鼠白血病病毒诱发的肿瘤组织，是科研上最常用的炎症细胞模型之一。该细胞易于增殖，高效DNA转染力，对RNA干扰敏感，常用作转染宿主细胞和复制小鼠诺如病毒。该细胞表面sIg-、Ia-、Thy-1.2抗原阴性。据报道，该细胞建系后已不分泌且检测不到病毒颗粒，XC斑点形成实验阴性。可以抗体依赖性地分解绵羊红血球与肿瘤靶细胞，LPS或PPD处理2天后可诱导分解红血球但对肿瘤靶细胞无作用。经检测鼠痘病毒阴性。
质量控制：
1.支原体检测结果：每个批次均通过本库支原体检测，结果为阴性。
2.STR鉴定结果：①该株细胞DNA进行小鼠细胞STR分型结果显示，扩增后图谱清晰，分型结果良好：1-1：16；1-2:17；2-1:16；3-2：14；4-2:22.3；5-5:14；6-4:17,18；6-7：12；7-1:25.2；8-1:13；11-2:17；12-1:16；13-1：16.2；15-3:22.3；17-2:14,16；18-3:18；19-2:14；X-1:24。②该株细胞确为小鼠细胞，没有人源细胞污染。 |
动物种别
Organism |
小鼠 |
性别
Gender |
雄性 |
组织来源
Tissue and Cell Type |
Abelson鼠科白血病病毒诱导的肿瘤；腹水 |
形态
Morphology |
单核细胞样/巨噬细胞样，贴壁生长 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
小鼠白血病病毒诱发肿瘤巨噬细胞RAW 264.7完全培养液配方(100 ml)：
DMEM (Invitrogen, 11960-044) 88 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml
FBS (Gibco) 10 ml
气相：空气95%，二氧化碳5%；37℃培养。
参考传代周期：3-5天
参考传代比例：1：3-1：6
参考换液频率：2-3天
冻存液配方：完全培养液95%，DMSO 5%
生物安全等级：BSL-2
注意事项：
(1)该细胞传代时不需要用胰酶消化。传代时，吸走部分培养液，留下少许培养液(例如使用T75培养瓶则留下10 ml)，用无菌细胞刮刮拭培养表面将细胞刮落，充分吹打后接种到新的装有新鲜培养液+的培养瓶内，混匀。
(2)在正常培养条件下，未经诱导分化的RAW264.7细胞形态上呈现松散贴壁的纺锤形和圆形或者立方形。当细胞密度较大时，细胞会轻微脱落变圆或者许多细胞堆积在一起，有些细胞甚至脱落漂浮。这些漂浮的细胞是存活的，在传代时应收集起来，离心后细胞沉淀可以继续培养。
(3)血清质量差异可能引起细胞贴壁能力变化，应选用高质量的胎牛血清。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198Denlinger LC, et al. Regulation of inducible nitric oxide synthase expression by macrophage purinoreceptors and calcium. J. Biol. Chem. 271: 337-342, 1996. PubMed: 8550583
Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116
Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776
Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306
Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609
Lokuta MA, et al. Mechanisms of murine RANTES chemokine gene induction by newcatle disease virus. J. Biol. Chem. 271: 13731-13738, 1996. PubMed: 8662857
Taylor MF, et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA. J. Biol. Chem. 271: 17445-17452, 1996. PubMed: 8663413
Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.
Hartley JW, et al. Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line. Retrovirology. 4: 5:1, 2008. PubMed 18177500.
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