半岛bd体育手机客户端
介绍 目录号
Serial | TCM35 | 标识符
Identifier | CSTR:19375.09.3101MOUTCM35 | 描述
Description | 这是一株肾脏-2α细胞株的非回复8-氮杂鸟嘌呤抗性(HPRT-, HGPRT-)克隆。 测试发现缺肢畸形病毒(鼠痘)阴性。
在本库通过支原体检测。 | 动物种别
Organism | 小鼠 | 性别
Gender | *** | 组织来源
Tissue and Cell Type | 肾腺癌；肾 | 形态
Morphology | 变形虫样 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Klebe RJ , et al. Mapping of a human genetic regulator element by somatic cell genetic analysis. Proc. Natl. Acad. Sci. USA 66: 1220-1227, 1970. PubMed: 4920091Science 145: 709, 1964.Klebe RJ , et al. Controlled production of proliferating somatic cell hybrids. J. Cell Biol. 45: 74-82, 1970. PubMed: 4318843Felluga B , et al. Electron microscope observations on virus particles associated with a transplantable renal adenocarcinoma in BALB-cf-Cd mice. J. Natl. Cancer Inst. 43: 319-333, 1969. PubMed: 5797837Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
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