浜у搧浠嬬粛
| 鐩綍鍙饵br/>Serial |
GNM17 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101MOUGNM17 |
鎻忚堪
Description |
OP9缁嗚優鏍簮鑷柊鐢熺殑op/op 灏忛紶棰呯洊銆傚洜缂栫爜M-CSF鐨勫熀鍥犱腑鐨勪竴涓獊鍙橈紝瀹冧笉鑳界敓鎴愭湁鍔熻兘鐨勫法鍣粏鑳炲厠闅嗗埡婵€鍥犲瓙(M-CSF)銆侻-CSF鐨勫瓨鍦ㄥ鑳氳儙骞茬粏鑳?ES)鍒嗗寲鎴愯缁嗚優鑰屼笉鏄叾浠栧法鍣粏鑳炴湁鎶戝埗鍔熻兘銆 OP9缁嗚優鍙互鐢ㄤ簬涓庡皬榧犺儦鑳庡共缁嗚優鍏卞煿鍏讳互璇卞鑳氳儙骞茬粏鑳炲垎鍖栨垚鎴愮孩琛€鐞冩潵婧愮殑銆侀楂撴潵婧愮殑鍜孊缁嗚優璋辩郴鐨勮缁嗚優銆備笌OP9鍏卞煿鍏讳笉闇€瑕佸婧愮殑鐢熼暱鍥犲瓙鎴栧鏉傜殑鑳氳儙缁撴瀯銆傝繖涓郴缁熷鐮旂┒閫犺缁嗚優鐨勫彂鑲插拰鍒嗗寲鐨勫垎瀛愭満鐞嗘湁鐢ㄣ€侟br/>鍦ㄦ湰搴撻€氳繃鏀師浣撴娴嬨€侟/span> |
鍔ㄧ墿绉嶅埆
Organism |
灏忛紶 |
鎬у埆
Gender |
*** |
缁勭粐鏉ユ簮
Tissue and Cell Type |
楠ㄩ珦锛屽熀璐?/span> |
| 褰㈡€?br/>Morphology |
鎴愮氦缁寸粏鑳濣/span> |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
MEM伪-鍩瑰吇鍩?GIBCO,璐у彿12000022锛屾坊鍔燦aHCO3 1.5g/L锛岃倢閱 43.2mg/l , 鍙堕吀 8.82mg/l ,尾-宸熀涔欓唶 7.8mg/l )锛?0%锛涗紭璐ㄨ儙鐗涜娓咃紝20%銆侟br/>姘旂浉锛氱┖姘旓紝95%锛涗簩姘у寲纰筹紝5%銆侟br/>娓╁害锛?7鎽勬皬搴︺€侟/span> |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Nakano T , et al. Generation of lymphohematopoietic cells from embryonic stem cells in culture. Science 265: 1098-1101, 1994. PubMed: 8066449Nakano T , et al. In vitro development of primitive and definitive erythrocytes from different precursors. Science 272: 722-724, 1996. PubMed: 8614833Nakano T . Lymphohematopoietic development from embryonic stem cells in vitro. Semin. Immunol. 7: 197-203, 1995. PubMed: 7579206Motoyama N , et al. bcl-x prevents apoptotic cell death of both primitive and definitive erythrocytes at the end of maturation. J. Exp. Med. 189: 1691-1698, 1999. PubMed: 10359572Nakano T . In vitro development of hematopoietic system from mouse embryonic stem cells: a new approach for embryonic hematopoiesis. Int. J. Hematol. 65: 1-8, 1996. PubMed: 8990620Nakano T , et al. Development of erythroid cells from mouse embryonic stem cells in culture: potential use for erythroid transcription factor study. Leukemia 3: 496-500, 1997. PubMed: 9209437Suwabe N , et al. GATA-1 regulates growth and differentiation of definitive erythroid lineage cells during in vitro ES cell differentiation. Blood 92: 4108-4118, 1998. PubMed: 9834216Suzuki A , Nakano T . Development of hematopoietic cells from embryonic stem cells. Int. J. Hematol. 73: 1-5, 2001. PubMed: 11372743Eto K , et al. Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling. Proc. Natl. Acad. Sci. USA 99: 12819-12824, 2002. PubMed: 12239348 |
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鐩稿叧浜у搧
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鍦ㄧ嚎鐣欒█
