Oct4-Neo灏忛紶鑳氳儙骞茬粏鑳濣/title> - 半岛电竞官方网址

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Oct4-Neo灏忛紶鑳氳儙骞茬粏鑳濣/div>

Oct4-Neo灏忛紶鑳氳儙骞茬粏鑳炲浘鐗?></a></div> <span style=

绱㈠彇璧勬枡鍙婃姤浠饵/span>

浜у搧浠嬬粛

鐩綍鍙饵br/>Serial	SCSP-213
鏍囪瘑绗?br/>Identifier	CSTR:19375.09.3101MOUSCSP213
鎻忚堪 Description	甯ct4-Neo鍩哄洜鐨勫皬榧犲叏鑳芥€ц儦鑳庡共缁嗚優锛屾瘡鏀粏鑳為噺1脳10⁶銆傛瘡涓壒娆″潎閫氳繃鏈簱鏀師浣撴娴嬶紝缁撴灉涓洪槾鎬с€侟/span>
鍔ㄧ墿绉嶅埆 Organism	灏忛紶锛孋57BL/6J脳C57BL/6J鍝佺郴
鎬у埆 Gender
缁勭粐鏉ユ簮 Tissue and Cell Type	灏忛紶鑳氳儙鍐呯粏鑳炲洟
褰㈡€?br/>Morphology	鐞冨舰鍏嬮殕
鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions	mES瀹屽叏鍩瑰吇娑查厤鏂?600 ml)锛欬br/>DMEM (Invitrogen 12430) 497 ml ES绾BS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 渭l(缁堟祿搴︿负1000U/ml) 尾-Mer (Invitrogen 21985) 1.5 ml 姘旂浉锛氱┖姘旓紝95%锛涗簩姘у寲纰筹紝5%銆傛俯搴︼細37鎽勬皬搴︺€侟/span>
渚涘簲闄愬埗 Permits and Restrictions	A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span>
REFERENCE	Chen et al., Self-renewal of embryonic stem cells by a small molecule. PNAS, 103.No. 46. (2006), 17266鈥?7271

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