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介绍 目录号
Serial | SCSP-515 | 标识符
Identifier | CSTR:19375.09.3101MOUSCSP515 | 描述
Description | NIH/3T3是从小鼠NIH/Swiss品系的胚胎中建立的成纤维细胞株。该细胞易于转染，对肉瘤病毒转化灶形成和白血病病毒增殖十分敏感。据报道，鼠痘病毒(ectromelia virus，ECTV)检测结果为阴性。
细胞质控结果：
1.支原体检测结果：每个批次均通过本库支原体检测，结果为阴性。
2.STR鉴定结果：
①该株细胞DNA进行小鼠细胞STR分型结果显示，扩增后图谱清晰，分型结果良好: 1-1: 10；1-2: 13, 17；2-1: 9；3-2: 14, 15；4-2: 19.3, 20.3；5-5: 14, 15；6-4: 15.3；6-7: 12；7-1: 29；8-1: 15；11-2: 15, 17；12-1: 20；13-1: 16.2；15-3: 20.3；17-2: 13, 14；18-3: 17, 19；19-2: 11, 12；X-1: 25。
②该细胞与ExPASy中NIH 3T3细胞的STR数据匹配率为100%。
③该株细胞确为小鼠细胞，没有人源细胞污染。 | 动物种别
Organism | 小鼠，品系NIH/Swiss | 性别
Gender |
| 组织来源
Tissue and Cell Type | 胚胎 | 形态
Morphology | 成纤维细胞，贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | NIH/3T3 完全培养液 配方(100 ml)
DMEM (Invitrogen, 11960-044)88 ml
新生牛血清 (Gibco)灭活后使用 10 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
【注意事项：】
NIH/3T3细胞对于血清较为敏感，不能使用胎牛血清(FBS)培养该细胞。
生物安全等级：BSL-1
参考传代周期：2～3天
参考传代比例：1：4(备注：切勿使细胞生长过密，每周至少传代2次，保证细胞密度不超过80%。)
参考换液频率：2～3天
冻存液配方：完全培养液95%，DMSO 5% | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Jainchill JL, et al. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. J. Virol. 4: 549-553, 1969. PubMed: 4311790Andersson P, et al. A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfection. Cell 16: 63-75, 1979. PubMed:84715Copeland NG, Cooper GM. Transfection by exogenous and endogenous murine retrovirus DNAs. Cell 16: 347-356, 1979. PubMed: 222457Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256Jones PL, et al. Tumor necrosis factor alpha and interleukin-1beta regulate the murine manganese superoxide dismutase gene through a complex intronic enhancer involving C/EBP-beta and NF-kappaB. Mol. Cell. Biol. 17: 6970-6981, 1997. PubMed:9372929Gonzalez Armas JC, et al. DNA immunization confers protection against murine cytomegalovirus infection. J. Virol. 70: 7921-7928, 1996. PubMed: 8892915Siess DC, et al. Exceptional fusogenicity of chinese hamster ovary cells with murine retrovirus suggests roles for cellular factor(s) and receptor clusters in the membrane fusion process. J. Virol. 70: 3432-439, 1996. PubMed: 8648675Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649Medin JA, et al. Correction in trans for Fabry disease: expression, secretion, and uptake of alpha-galactosidase A in patient-derived cells driven by a high-titer recombinant retroviral vector. Proc. Natl. Acad. Sci. USA 93: 7917-7922, 1996. PubMed: 8755577Lee JH, et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812Chang K, Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591Cranmer LD, et al. Identification, analysis, and evolutionary relationships of the putative murine cytomegalovirus homologs of the human cytomegalovirus UL82 (pp71) and UL83 (pp65) matrix phosphoproteins. J. Virol. 70: 7929-7939, 1996. PubMed:8892916Shisler J, et al. Induction of susceptibility to tumor necrosis factor by E1A is dependent on binding to either p300 or p105-Rb and induction of DNA synthesis. J. Virol. 70: 68-77, 1996. PubMed:8523594Cavanaugh VJ, et al. Murine cytomegalovirus with a deletion of genes spanning HindIII-J and -I displays altered cell and tissue tropism. J. Virol. 70: 1365-1374, 1996. PubMed: 8627652Westerman KA, Leboulch P. Reversible immortalization of mammalian cells mediated by retroviral transfer and site-specific recombination. Proc. Natl. Acad. Sci. USA 93: 8971-8976, 1996. PubMed: 8799138The NIH/3T3, a continuous cell line of highly contact-inhibited cells was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 (ATCC CCL-92) and the inbred BALB/c 3T3 (ATCC CCL-163). The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. |
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