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细胞培养基 >> 细胞株
NIH/3T3小鼠胚胎成纤维细胞
NIH/3T3小鼠胚胎成纤维细胞图片
半岛bd体育手机客户端 介绍
目录号
Serial
GNM 6
标识符
Identifier
CSTR:19375.09.3101MOUGNM6
描述
Description
与原始的随机交配3T3和近交系BALB/c 3T3建株方法一样,NIH/3T3,是从NIH Swiss小鼠胚胎培养物中建立的高度接触抑制的连续细胞株。 为了培育在形态学特征上更适合于进行转化分析的亚株,建立的NIH/3T3细胞株又进行了五轮以上亚克隆。 这株细胞对DNA转化及转染研究十分有用。 检测表明肢骨发育畸形病毒(鼠痘)阴性。
在本库通过支原体检测。
动物种别
Organism
小鼠
性别
Gender
***
组织来源
Tissue and Cell Type
胚胎
形态
Morphology
成纤维细胞
培养基和添加剂
Complete Growth Medium and Culture Conditions
DMEM培养基(GIBCO by life technologies ,货号C11995500BT),90%;优质胎牛血清,10%。
气相:空气,95%;二氧化碳,5%。
温度:37摄氏度。
供应限制
Permits and Restrictions
A。仅供研究之用。
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