细胞培养基 >> 细胞株
Neuro-2a [N2a, Neuro-2a]小鼠脑神经瘤细胞
半岛bd体育手机客户端 介绍
目录号
Serial |
TCM29 |
标识符
Identifier |
CSTR:19375.09.3101MOUTCM29 |
描述
Description |
克隆Neuro-2A是由R.J.Klebe和F.H.Ruddle经A株白鼠的自生肿瘤建立。 该细胞产生大量微管蛋白，此蛋白在神经细胞中起应答轴浆流动的收缩系统作用。
在本库通过支原体检测。 |
动物种别
Organism |
小鼠 |
性别
Gender |
不明 |
组织来源
Tissue and Cell Type |
脑神经母细胞瘤，神经母细胞 |
形态
Morphology |
神经和阿米巴样干细胞，主要贴壁生长、较少部分半贴壁生长 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Olmsted JB , et al. Isolation of microtubule protein from cultured mouse neuroblastoma cells. Proc. Natl. Acad. Sci. USA 65: 129-136, 1970. PubMed: 5263744Klebe RJ , Ruddle FH . Neuroblastoma: Cell culture analysis of a differentiating stem cell system. J. Cell Biol. 43: 69A, 1969.Naslavsky N , et al. Characterization of detergent-insoluble complexes containing the cellular prion protein and its scrapie isoform. J. Biol. Chem. 272: 6324-6331, 1997. PubMed: 9045652Kaneko K , et al. Evidence for protein X binding to a discontinuous epitope on the cellular prion protein during scrapie prion propagation. Proc. Natl. Acad. Sci. USA 94: 10069-10074, 1997. PubMed: 9294164Maestrini E , et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614 |
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