浜у搧浠嬬粛
| 鐩綍鍙饵br/>Serial |
SCSP-522 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101MOUSCSP522 |
鎻忚堪
Description |
姝ょ粏鑳炴牚鏄埄鐢↙-M(TK-)缁嗚優杞煋Wnt-3A琛ㄨ揪杞戒綋锛屽苟鍦ㄥ惈G418鐨勫煿鍏诲熀涓瓫閫夎幏寰楃殑绋宠浆鏍€俉nt-3A鍩哄洜缂栫爜涓€涓叿鏈夊箍娉涗俊鍙峰姛鑳界殑鍒嗘硨鎬х硸铔嬬櫧锛岃€學nt鍩哄洜鎺у埗鑳氳儙鍙戣偛杩囩▼涓澶氭ā寮忓缓鎴愬拰鐢熼暱浜嬩欢銆傛缁嗚優鍒嗘硨鏈夌敓鐗╂椿鎬х殑Wnt-3A铔嬬櫧锛屾槸鐩墠鐢熶骇Wnt-3A鏉′欢鍩瑰吇鍩虹殑**鏉ユ簮銆傜敱浜庤繖绉嶆潯浠跺煿鍏诲熀鍚湁Wnt-3A铔嬬櫧浠ュ鐨勫叾瀹冨洜瀛愶紝瀵逛簬娑夊強Wnt-3A鏉′欢鍩瑰吇鍩虹殑瀹為獙鏈夊繀瑕佺敤鍏朵翰鏈粏鑳炴牚(L缁嗚優)浣滀负瀵圭収缁勩€侟br/>缁嗚優璐ㄦ帶缁撴灉锛欬br/>1.鏀師浣撴娴嬬粨鏋滐細姣忎釜鎵规鍧囬€氳繃鏈簱鏀師浣撴娴嬶紝缁撴灉涓洪槾鎬с€侟br/>2.STR閴村畾缁撴灉锛欬br/>鈶犺鏍粏鑳濪NA杩涜灏忛紶缁嗚優STR鍒嗗瀷缁撴灉鏄剧ず锛屾墿澧炲悗鍥捐氨娓呮櫚锛屽垎鍨嬬粨鏋滆壇濂斤細1-1锛?0锛?-2锛?7, 18锛?-1锛?锛?-2锛?4, 15锛?-2锛?0.3锛?-5锛?4锛?-4锛?7, 19锛?-7锛?2锛?-1锛?5, 26锛?-1锛?6锛?1-2锛?5, 16锛?2-1锛?6锛?3-1锛?7.1锛?5-3锛?4.3, 25.3锛?7-2锛?4, 15锛?8-3锛?4.3, 16锛?9-2锛?2锛沊-1锛?6, 27銆侟br/>鈶¤鏍粏鑳炵‘涓哄皬榧犵粏鑳烇紝娌℃湁浜烘簮缁嗚優姹℃煋銆侟/span> |
鍔ㄧ墿绉嶅埆
Organism |
灏忛紶锛孋3H/An鍝佺郴 |
鎬у埆
Gender |
闆勬€э紝100鏃ラ緞 |
缁勭粐鏉ユ簮
Tissue and Cell Type |
鐨笅缁撶紨缁勭粐锛涗钩鏅曞拰鑴傝偑 |
| 褰㈡€?br/>Morphology |
鎴愮氦缁寸粏鑳烇紝璐村鐢熼暱 |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
灏忛紶鐨笅缁撶紨缁勭粐缁嗚優L Wnt-3A 瀹屽叏鍩瑰吇娑查厤鏂?100 ml)锛欬br/>DMEM (Invitrogen, 11960044) 88 ml
FBS (Gibco) 10 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate 100 mM Solution(invitrogen 11360070) 1 ml
G418(Gibco,10131027) 缁堟祿搴?.4 mg/ml
鐢熺墿瀹夊叏绛夌骇锛欱SL-1
鍙傝€冧紶浠ｅ懆鏈燂細2-3澶?br/>鍙傝€冧紶浠ｆ瘮渚嬶細1:3-1:5
鍙傝€冩崲娑查鐜囷細2-3澶?br/>鍐诲瓨娑查厤鏂癸細瀹屽叏鍩瑰吇娑?5%锛孌MSO 5%
銆愬娉ㄣ€慄br/>璇ョ粏鑳炶创澹佽緝鎱紝涓€鑸鑻忓悗闇€24锝?8灏忔椂鎵嶈兘璐村瀹屽叏銆侟/span> |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Willert K, et al. Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature 423: 448-452, 2003. PubMed:12717451 |
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鐩稿叧浜у搧
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鍦ㄧ嚎鐣欒█
