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细胞培养基 >> 细胞株
J1小鼠胚胎干细胞
J1小鼠胚胎干细胞图片
半岛bd体育手机客户端 介绍
目录号
Serial
SCSP-219
标识符
Identifier
CSTR:19375.09.3101MOUSCSP219
描述
Description
J1细胞来源于雄性鼠灰色的129S4/SvJae胚胎。该细胞能进入生殖系。每个批次均通过本库支原体检测,结果为阴性。
培养时需使用CF-1 MEF(SCSP-105R)作为饲养层细胞。
动物种别
Organism
小鼠,129S4/SvJae品系
性别
Gender
组织来源
Tissue and Cell Type
小鼠胚胎内细胞团
形态
Morphology
球形克隆,贴壁生长
培养基和添加剂
Complete Growth Medium and Culture Conditions
mES完全培养液配方(600 ml):
DMEM (Invitrogen 12430) 497 ml
ES级FBS (biochrom S4115) 90 ml
Glutamax (Invitrogen 35050) 6 ml
NEAA (Invitrogen 11140) 6 ml
LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml)
β-Mer (Invitrogen 21985) 1.5 ml
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCE Stevens LC. A new inbred subline of mice (129-terSv) with a high incidence of spontaneous congenital testicular teratomas. J. Natl. Cancer Inst. 50: 235-242, 1973. PubMed: 4692863Li E, et al. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 69: 915-926, 1992. PubMed: 1606615 Krzyzanowski PM, et al. Identification of novel stem cell markers using gap analysis of gene expression data. Genome Biology 8(9): R193, 2007. PubMed: 17875203Luikenhuis S, et al. Antisense transcription through the Xist locus mediates Tsix function in embryonic stem cells. Mol. Cell Biol. 21(24): 8512?8520, 2001. PubMed: 11713286Goto T, et al. Regulation of X-Chromosome inactivation in development in mice and humans. Micro. Molec. Biol. Rev. (ASM) 62(2): 362-378, 1998. PubMed: 9618446Yap DYL, et al. Using biomarker signature patterns for an mRNA molecular diagnostic of mouse embryonic stem cell differentiation state. BMC Genomics 8: 210, 2007. PubMed: 17605829Haase VH, et al. Vascular tumors in livers with targeted inactivation of the von Hippel?Lindau tumor suppressor. Proc. Natl. Acad. Sci. 98(4): 1583?1588, 2001. PubMed: 11171994Oda M, et al. DNA methylation regulates long-range gene silencing of an X-linked homeobox gene cluster in a lineage-specific manner. Genes Dev. 20(24): 3382-3394, 2006. PubMed: 17182866Lorincz MC, et al. DNA methylation density influences the stability of an epigenetic imprint and Dnmt3a/b-independent de novo methylation. Mol. Cell Biol. 22(21): 7572?7580, 2002.PubMed: 12370304Tucker KL, et al. A transgenic mouse strain expressing four drug-selectable marker genes. Nucleic Acids Res. 25: 3745-3746, 1997. PubMed: 9278500Biniszkiewicz D, et al. Dnmt1 overexpression causes genomic hypermethylation, loss of imprinting, and embryonic lethality. Mol. Cell Biol. 22(7): 2124-2135, 2002. PubMed: 11884600
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