浜у搧浠嬬粛
| 鐩綍鍙饵br/>Serial |
SCSP-219 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101MOUSCSP219 |
鎻忚堪
Description |
J1缁嗚優鏉ユ簮浜庨泟鎬ч紶鐏拌壊鐨?29S4/SvJae鑳氳儙銆傝缁嗚優鑳借繘鍏ョ敓娈栫郴銆傛瘡涓壒娆″潎閫氳繃鏈簱鏀師浣撴娴嬶紝缁撴灉涓洪槾鎬с€侟br/>鍩瑰吇鏃堕渶浣跨敤CF-1 MEF(SCSP-105R)浣滀负楗插吇灞傜粏鑳炪€侟/span> |
鍔ㄧ墿绉嶅埆
Organism |
灏忛紶锛?29S4/SvJae鍝佺郴 |
鎬у埆
Gender |
闆凕/span> |
缁勭粐鏉ユ簮
Tissue and Cell Type |
灏忛紶鑳氳儙鍐呯粏鑳炲洟 |
| 褰㈡€?br/>Morphology |
鐞冨舰鍏嬮殕锛岃创澹佺敓闀军/span> |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
mES瀹屽叏鍩瑰吇娑查厤鏂?600 ml)锛欬br/>DMEM (Invitrogen 12430) 497 ml
ES绾BS (biochrom S4115) 90 ml
Glutamax (Invitrogen 35050) 6 ml
NEAA (Invitrogen 11140) 6 ml
LIF (Millipore ESG1107) 60 渭l(缁堟祿搴︿负1000U/ml)
尾-Mer (Invitrogen 21985) 1.5 ml
姘旂浉锛氱┖姘旓紝95%锛涗簩姘у寲纰筹紝5%銆傛俯搴︼細37鎽勬皬搴︺€侟/span> |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Stevens LC. A new inbred subline of mice (129-terSv) with a high incidence of spontaneous congenital testicular teratomas. J. Natl. Cancer Inst. 50: 235-242, 1973. PubMed: 4692863Li E, et al. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 69: 915-926, 1992. PubMed: 1606615 Krzyzanowski PM, et al. Identification of novel stem cell markers using gap analysis of gene expression data. Genome Biology 8(9): R193, 2007. PubMed: 17875203Luikenhuis S, et al. Antisense transcription through the Xist locus mediates Tsix function in embryonic stem cells. Mol. Cell Biol. 21(24): 8512?8520, 2001. PubMed: 11713286Goto T, et al. Regulation of X-Chromosome inactivation in development in mice and humans. Micro. Molec. Biol. Rev. (ASM) 62(2): 362-378, 1998. PubMed: 9618446Yap DYL, et al. Using biomarker signature patterns for an mRNA molecular diagnostic of mouse embryonic stem cell differentiation state. BMC Genomics 8: 210, 2007. PubMed: 17605829Haase VH, et al. Vascular tumors in livers with targeted inactivation of the von Hippel?Lindau tumor suppressor. Proc. Natl. Acad. Sci. 98(4): 1583?1588, 2001. PubMed: 11171994Oda M, et al. DNA methylation regulates long-range gene silencing of an X-linked homeobox gene cluster in a lineage-specific manner. Genes Dev. 20(24): 3382-3394, 2006. PubMed: 17182866Lorincz MC, et al. DNA methylation density influences the stability of an epigenetic imprint and Dnmt3a/b-independent de novo methylation. Mol. Cell Biol. 22(21): 7572?7580, 2002.PubMed: 12370304Tucker KL, et al. A transgenic mouse strain expressing four drug-selectable marker genes. Nucleic Acids Res. 25: 3745-3746, 1997. PubMed: 9278500Biniszkiewicz D, et al. Dnmt1 overexpression causes genomic hypermethylation, loss of imprinting, and embryonic lethality. Mol. Cell Biol. 22(7): 2124-2135, 2002. PubMed: 11884600 |
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鐩稿叧浜у搧
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