| 鐩綍鍙饵br/>Serial |
SCSP-222 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101MOUSCSP222 |
鎻忚堪
Description |
閫氳繃灏嗙豢鑹茶崸鍏夎泲鐧?GFP)鎻掑叆鍒癘lig2鍩哄洜(涓€绉嶈氨绯荤壒寮傛€х殑杞綍鍥犲瓙)涓紝鏋勫缓鑾峰緱G-Olig2缁嗚優銆傚湪璇ョ粏鑳炵缁忓垎鍖栫殑娲荤粏鑳炰腑锛屽彲浠ヨ瀵熷埌GFP琛ㄨ揪銆傛瘡涓壒娆″潎閫氳繃鏈簱鏀師浣撴娴嬶紝缁撴灉涓洪槾鎬с€侟/span> |
鍔ㄧ墿绉嶅埆
Organism |
灏忛紶锛?29X1/SvJ鍝佺郴 |
鎬у埆
Gender |
|
缁勭粐鏉ユ簮
Tissue and Cell Type |
灏忛紶鑳氳儙鍐呯粏鑳炲洟 |
| 褰㈡€?br/>Morphology |
鐞冨舰鍏嬮殕 |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
mES瀹屽叏鍩瑰吇娑查厤鏂?600 ml)锛欬br/>DMEM (Invitrogen 12430) 497 ml
ES绾BS (biochrom S4115) 90 ml
Glutamax (Invitrogen 35050) 6 ml
NEAA (Invitrogen 11140) 6 ml
LIF (Millipore ESG1107) 60 渭l(缁堟祿搴︿负1000U/ml)
尾-Mer (Invitrogen 21985) 1.5 ml
姘旂浉锛氱┖姘旓紝95%锛涗簩姘у寲纰筹紝5%銆傛俯搴︼細37鎽勬皬搴︺€侟/span> |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Matise M, et al., Production of targeted embryonic stem cell clones. In: Matise M, et al., Gene Targeting: A Practical Approach, Oxford, Oxford University Press, 101-132, 1999Xian HQ, et al. A subset of ES-cell-derived neural cells marked by gene targeting. Stem Cells 21: 41-49, 2003. PubMed: 12529550 |
鍦ㄧ嚎鐣欒█
