缁嗚優鍩瑰吇鍩 >> 缁嗚優鏍
ES-E14TG2a灏忛紶鑳氳儙骞茬粏鑳濣/div>
浜у搧浠嬬粛
| 鐩綍鍙饵br/>Serial |
SCSP-204 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101MOUSCSP204 |
鎻忚堪
Description |
璇ョ粏鑳炵己涔廐GPRT(HPRT)锛屽苟涓斿0.06 mM鐨?-纭笩鍢屽懁鏈夋姉鎬с€傚綋鍦ㄩゲ鍏诲眰(鑳氳儙鎴愮氦缁寸粏鑳炴垨STO缁嗚優)涓婂煿鍏绘椂锛岀粏鑳炰繚鎸佹湭鍒嗗寲鐘舵€併€傚湪娌℃湁楗插吇灞傜殑鎯呭喌涓嬶紝缁嗚優鑷彂鍒嗗寲骞跺舰鎴愯儦鑳庣粨鏋勩€傚綋娉ㄥ叆鑳氭场涓椂锛岀粏鑳炲彲浠ヨ繘鍏ョ敓娈栫郴銆傚湪甯歌鐨勫垎瀛愬熀鍥犱慨楗版妧鏈箣鍚庯紝瀹冧滑鍙敤浜庨噸鏋勫皬榧犺儦鑳庛€傛瘡涓壒娆″潎閫氳繃鏈簱鏀師浣撴娴嬶紝缁撴灉涓洪槾鎬с€傛垜搴撳喕瀛樻椂涓篜8浠ｃ€侟br/>鍩瑰吇鏃堕渶浣跨敤鏄嗘槑鐧組EF(SCSP-101R)浣滀负楗插吇灞傜粏鑳炪€侟/span> |
鍔ㄧ墿绉嶅埆
Organism |
灏忛紶锛?29/Ola鍝佺郴 |
鎬у埆
Gender |
|
缁勭粐鏉ユ簮
Tissue and Cell Type |
灏忛紶鑳氳儙鍐呯粏鑳炲洟 |
| 褰㈡€?br/>Morphology |
鐞冨舰锛岃创澹佺敓闀军/span> |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
mES瀹屽叏鍩瑰吇娑查厤鏂?600 ml)锛欬br/>DMEM(Invitrogen 12430054)497 ml
ES绾BS (Gibco) 90 ml
Glutamax(Invitrogen 35050061) 6 ml
NEAA(Invitrogen 11140050) 6 ml
LIF(Millipore ESG1107) 60 渭l(缁堟祿搴︿负1000U/ml)
尾-Mer(Invitrogen 21985) 1.5 ml
姘旂浉锛氱┖姘旓紝95%锛涗簩姘у寲纰筹紝5%銆傛俯搴︼細37鎽勬皬搴︺€侟/span> |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Smith AG, Hooper ML. Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9, 1987. PubMed: 3569655Kuehn MR, et al. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326: 295-298, 1987. PubMed: 3029599Doetschman T, et al. Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330: 576-578, 1987. PubMed: 3683574 |
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鐩稿叧浜у搧
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