半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-202 |
标识符
Identifier |
CSTR:19375.09.3101MOUSCSP202 |
描述
Description |
在没有饲养层或条件培养基的情况下，ES-D3 [D3]细胞会自发分化为胚胎结构。可以将它们注射回囊胚并能进入生殖系。未分化的D3细胞可以通过基因靶向技术进行遗传修饰。每个批次均通过本库支原体检测，结果为阴性。
培养时需使用昆明白MEF(SCSP-101R)作为饲养层细胞。冻存管细胞为P12。 |
动物种别
Organism |
小鼠，129/Sv+c/+p品系 |
性别
Gender |
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组织来源
Tissue and Cell Type |
小鼠囊胚内细胞团 |
形态
Morphology |
球形克隆，贴壁生长 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
mES完全培养液配方(600 ml)：
DMEM (Invitrogen 12430) 497 ml
ES级FBS (Gibco) 90 ml
Glutamax (Invitrogen 35050-061) 6 ml
NEAA (Invitrogen 11140-050) 6 ml
LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml)
β-Mer (Invitrogen 21985) 1.5 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Doetschman TC, et al. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J. Embryol. Exp. Morphol. 87: 27-45, 1985. PubMed: 3897439Williams RL, et al. Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature 336: 684-687, 1988. PubMed: 3143916Gossler A, et al. Transgenesis by means of blastocyst-derived embryonic stem cell lines. Proc. Natl. Acad. Sci. USA 83: 9065-9069, 1986. PubMed: 3024164Doetschman T, et al. Targeted mutation of the Hprt gene in mouse embryonic stem cells. Proc. Natl. Acad. Sci. USA 85: 8583-8587, 1988. PubMed: 3186749Chambon P, et al. Genetically engineered mice containing alterations in the genes encoding retinoic acid receptor proteins. US Patent 6,486,381 dated Nov 26 2002 |
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