半岛bd体育手机客户端 介绍
目录号
Serial |
TCM41 |
标识符
Identifier |
CSTR:19375.09.3101MOUTCM41 |
描述
Description |
EL4是从用9,10-二甲基-1,2-苯并蒽在C57BL小鼠中诱导的淋巴瘤中建立的。 能抗0.1 mM 氢化可的松，对20 mcg/ml PHA敏感。 还有一个亚株(EL4.IL-2)可以生成高水平的IL-2。 检测表明肢骨发育畸形病毒(鼠痘)阴性。
在本库通过支原体检测。 |
动物种别
Organism |
小鼠 |
性别
Gender |
*** |
组织来源
Tissue and Cell Type |
T 淋巴细胞；淋巴瘤 |
形态
Morphology |
淋巴母细胞 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，90%；马血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Old LJ, et al. The G (Gross) leukemia antigen. Cancer Res. 25: 813-819, 1965. PubMed: 4284252 Gorer PA. Studies in antibody response of mice to tumour inoculation. Br. J. Cancer 4: 372-379, 1950. PubMed: 14801344 Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 Shevach EM, et al. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J. Immunol. 108: 1146-1151, 1972. PubMed: 4112916 Aparicio CL, et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 9421636 Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019 |
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