半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-220 |
标识符
Identifier |
CSTR:19375.09.3101MOUSCSP220 |
描述
Description |
小鼠全能性胚胎干细胞，每支细胞量1×106。每个批次均通过本库支原体检测，结果为阴性。
培养时需使用CF-1 MEF(SCSP-105R)作为饲养层细胞。 |
动物种别
Organism |
小鼠，129S5/SvEvTac品系 |
性别
Gender |
雄 |
组织来源
Tissue and Cell Type |
小鼠胚胎内细胞团 |
形态
Morphology |
球形克隆 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
mES完全培养液配方(600 ml)：
DMEM (Invitrogen 12430) 497 ml
ES级FBS (biochrom S4115) 90 ml
Glutamax (Invitrogen 35050) 6 ml
NEAA (Invitrogen 11140) 6 ml
LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml)
β-Mer (Invitrogen 21985) 1.5 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Festing MF, et al. Revised nomenclature for strain 129 mice. Mamm. Genome. 10(8):836, 1999. PubMed: 10430671Simpson EM, et al. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat. Genet. 16(1):19-27, 1997. PubMed: 9140391Threadgill DW, et al. Genealogy of the 129 inbred strains: 129/SvJ is a contaminated inbred strain. Mamm. Genome. 8(6):390-393 1997. PubMed: 9166580Auerbach W, et al. Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines. BioTechniques 29: 1024-1028, 1030, 1032, 2000. PubMed: 11084865 |
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