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细胞培养基 >> 细胞株
C57BL/6小鼠胚胎干细胞
C57BL/6小鼠胚胎干细胞图片
半岛bd体育手机客户端 介绍
目录号
Serial
SCSP-218
标识符
Identifier
CSTR:19375.09.3101MOUSCSP218
描述
Description
C57BL/6植入c2J囊胚时,毛色嵌合体的得率高;而植入FVB囊胚产生的嵌合体数量少。ES细胞可以进入FVB/NJ(FVB)和同源突变品系C57BL/6-Tyrc-2J(c2J)这两个供体宿主囊胚的生殖系。每个批次均通过本库支原体检测,结果为阴性。
培养时需使用CF-1 MEF(SCSP-105R)作为饲养层细胞。冻存时为P26
动物种别
Organism
小鼠,C57BL/6品系
性别
Gender
组织来源
Tissue and Cell Type
小鼠胚胎内细胞团
形态
Morphology
球形克隆,贴壁生长
培养基和添加剂
Complete Growth Medium and Culture Conditions
mES完全培养液配方(600 ml):
DMEM (Invitrogen 12430) 497 ml
ES级FBS (biochrom S4115) 90 ml
Glutamax (Invitrogen 35050) 6 ml
NEAA (Invitrogen 11140) 6 ml
LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml)
β-Mer (Invitrogen 21985) 1.5 ml
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCE Brook FA, et al. The derivation of highly germline-competent embryonic stem cells containing NOD-derived genome. Diabetes. 52:205-208, 2003. PubMed: 12502514Brook FA, Gardner RL. The origin and efficient derivation of embryonic stem cells in the mouse. Proc. Natl. Acad. Sci. USA. 94: 5709-5712, 1997. PubMed: 9159137 Schuster-Gossler K, et al. Use of coisogenic host blastocysts for efficient establishment of germline chimeras with C57BL/6J ES cell lines. BioTechniques 31: 1022-1024, 1026, 2001. PubMed: 11730008 Auerbach W, et al. Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines. BioTechniques 29: 1024-1028, 1030, 1032, 2000. PubMed: 11084865 Matise M, et al., Production of targeted embryonic stem cell clones. In: Matise M, et al., Gene Targeting: A Practical Approach.Oxford, Oxford University Press, 101-132, 1999Lemckert FA, et al. Gene targeting in C57BL/6 ES cells. Successful germ line transmission using recipient BALB/c blastocysts developmentally matured in vitro. Nucleic Acids Res. 25: 917-918, 1997. PubMed: 9016649 Ledermann B, Burki K. Establishment of a germ-line competent C57BL/6 embryonic stem cell line. Exp. Cell Res. 197: 254-258, 1991. PubMed: 1959560
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