细胞培养基 >> 细胞株
C3H/10T1/2, Clone 8小鼠胚胎成纤维细胞
半岛bd体育手机客户端 介绍
目录号
Serial |
GNM19 |
标识符
Identifier |
CSTR:19375.09.3101MOUGNM19 |
描述
Description |
该细胞系是由C. Reznikoff等(1972)从C3H系小鼠胚胎细胞分离。此细胞对过度长满的细胞有丝分裂抑制非常敏感，在同源小鼠中不产生肿瘤，无自然转化的背景，也不含明显内源性转化鼠类白血病或肉瘤病毒。
在本库通过支原体检测。 |
动物种别
Organism |
小鼠 |
性别
Gender |
未知 |
组织来源
Tissue and Cell Type |
胚胎 |
形态
Morphology |
成纤维细胞 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Reznikoff CA , et al. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33: 3239-3249, 1973. PubMed: 4796800Terzaghi M , Little JB . Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253: 548-549, 1975. PubMed: 1167940Mondal S , Heidelberger C . Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature 260: 710-711, 1976. PubMed: 1264242Smith GJ , et al. Clonal analysis of the expression of multiple transformation phenotypes and tumorigenicity by morphologically transformed 10T1/2 cells. Cancer Res. 53: 500-508, 1993. PubMed: 8425183Rapp UR , et al. Endogenous oncornaviruses in chemically induced transformation. I. Transformation independent of virus production. Virology 65: 392-409, 1975. PubMed: 165619Reznikoff CA , et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355Jain MK , et al. Molecular cloning and characterization of SmLIM, a developmentally regulated LIM protein preferentially expressed in aortic smooth muscle cells. J. Biol. Chem. 271: 10194-10199, 1996. PubMed: 8626582 |
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