细胞培养基 >> 细胞株
C2C12小鼠胚胎肌母细胞(2014年新引进)(未分化,可诱导分化形成肌纤维)
半岛bd体育手机客户端 介绍
目录号 Serial |
SCSP-505 |
标识符 Identifier |
CSTR:19375.09.3101MOUSCSP505 |
描述 Description |
这株细胞是D. Yaffe和O. Saxel建立的小鼠成肌细胞株的亚克隆(由H. Blau等人构建)。C2C12细胞株可在低血清饥饿下分化,形成可伸缩的肌管并表达特征性的肌蛋白。如用骨形成蛋白2(BMP-2)处理后,分化途径从成肌细胞转换成成骨细胞。检测表明鼠痘病毒(ectromelia virus,ECTV)阴性。该细胞复苏后不耐受长时间运输,邮寄后会有细胞脱落、坏死等现象,有条件请选用干冰冷链快递或复苏后上门自取。 细胞质控结果: 1.支原体检测结果:每个批次均通过本库支原体检测,结果为阴性。 2.STR鉴定结果: ①该株细胞DNA进行小鼠细胞STR分型结果显示,扩增后图谱清晰,分型结果良好:1-1:10;1-2:16;2-1:9;3-2:14;4-2:19.3;5-5:15;6-4:18;6-7:12;7-1:26;8-1:17;11-2:16;12-1:16;13-1:17.1;15-3:25.3;17-2:15;18-3:16;19-2:12;X-1:25, 26。 ②该株细胞确为小鼠细胞,没有人源细胞污染。 3.诱导分化情况:通过本库诱导分化测试,在2%马血清刺激下可诱导分化形成肌纤维。 |
动物种别 Organism |
小鼠,C3H品系 |
性别 Gender |
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组织来源 Tissue and Cell Type |
肌肉 |
形态 Morphology |
成肌细胞样,贴壁生长 |
培养基和添加剂 Complete Growth Medium and Culture Conditions |
C2C12完全培养液配方(100 ml) DMEM Medium (Invitrogen, 11960-044 ) 88 ml FBS (Gibco) 10 ml Glutamax (Invitrogen, 35050061) 1 ml Sodium Pyruvate 100 mM Solution (invitrogen, 11360070) 1 ml 气相:空气,95%;二氧化碳,5%。温度:37摄氏度。 备注:C2C12细胞生长较快,培养时细胞密度不能过高, 并应及时更换培养液,否则容易 分化 生物安全等级:BSL-1 参考传代周期:2-3天 参考传代比例:根据细胞数量决定, 细胞密度应控制在 5000 – 15000 活细胞/平方厘米 参考换液频率:每2-3天换液 冷冻液配方:完全培养液95%,DMSO 5% |
供应限制 Permits and Restrictions |
A。仅供研究之用。 |
REFERENCE |
Yaffe D, Saxel O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270: 725-727, 1977. PubMed: 563524 Blau HM, et al. Plasticity of the differentiated state. Science 230: 758-766, 1985. PubMed: 2414846 Katagiri T, et al. Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]. J. Cell Biol. 127: 1755-1766, 1994. PubMed: 7798324 Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336 Kessler PD, et al. Gene delivery to skeletal muscle results in sustanined expression and systemic delivery of a therapeutic protein. Proc. Natl. Acad. Sci. USA 93: 14082-14087, 1996. PubMed: 8943064 Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936 Qing Y, et al. Inhibitory effects of iron on bone morphogenetic protein 2-induced osteoblastogenesis. J. Bone Miner. Res. 26(6): 1188-1196, 2011. PubMed: 21308772 |
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