浜у搧浠嬬粛
| 鐩綍鍙饵br/>Serial |
GNM26 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101MOUGNM26 |
鎻忚堪
Description |
杩欐槸D. Yaffe 鍜 O. Saxel寤虹珛鐨勫皬榧犳垚鑲岀粏鑳炴牚鐨勪簹鍏嬮殕(鐢盚. Blau绛変汉鏋勫缓)銆侰2C12缁嗚優鏍垎鍖栧緢蹇紝褰㈡垚鍙几缂╃殑鑲岀骞剁敓鎴愮壒寰佹€х殑鑲岃泲鐧姐€ 鐢ㄩ褰㈡垚铔嬬櫧2(BMP-2)澶勭悊锛屽鑷村垎鍖栭€斿緞浠庢垚鑲岀粏鑳炶浆鎹㈡垚鎴愰缁嗚優銆
妫€娴嬭〃鏄庤偄楠ㄥ彂鑲茬暩褰㈢梾姣?榧犵棙)闃存€с€侟br/>鍦ㄦ湰搴撻€氳繃鏀師浣撴娴嬨€侟/span> |
鍔ㄧ墿绉嶅埆
Organism |
灏忛紶 |
鎬у埆
Gender |
*** |
缁勭粐鏉ユ簮
Tissue and Cell Type |
缁勭粐锛 鑲岃倝鍝佺郴锛 C3H缁嗚優绫诲瀷锛 鎴愯倢缁嗚優銆侟/span> |
| 褰㈡€?br/>Morphology |
鎴愯倢缁嗚優 |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
DMEM鍩瑰吇鍩?GIBCO by life technologies ,璐у彿C11995500BT)锛?0%锛涗紭璐ㄨ儙鐗涜娓咃紝10%銆侟br/>姘旂浉锛氱┖姘旓紝95%锛涗簩姘у寲纰筹紝5%銆侟br/>娓╁害锛?7鎽勬皬搴︺€侟/span> |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Yaffe D, Saxel O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270: 725-727, 1977. PubMed: 563524Blau HM, et al. Plasticity of the differentiated state. Science 230: 758-766, 1985. PubMed: 2414846Katagiri T, et al. Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]. J. Cell Biol. 127: 1755-1766, 1994. PubMed: 7798324Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336Kessler PD, et al. Gene delivery to skeletal muscle results in sustanined expression and systemic delivery of a therapeutic protein. Proc. Natl. Acad. Sci. USA 93: 14082-14087, 1996. PubMed: 8943064Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936 |
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鐩稿叧浜у搧
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鍦ㄧ嚎鐣欒█
