半岛bd体育手机客户端 介绍
目录号
Serial |
GNM26 |
标识符
Identifier |
CSTR:19375.09.3101MOUGNM26 |
描述
Description |
这是D. Yaffe 和 O. Saxel建立的小鼠成肌细胞株的亚克隆(由H. Blau等人构建)。C2C12细胞株分化很快，形成可伸缩的肌管并生成特征性的肌蛋白。 用骨形成蛋白2(BMP-2)处理，导致分化途径从成肌细胞转换成成骨细胞。
检测表明肢骨发育畸形病毒(鼠痘)阴性。
在本库通过支原体检测。 |
动物种别
Organism |
小鼠 |
性别
Gender |
*** |
组织来源
Tissue and Cell Type |
组织： 肌肉品系： C3H细胞类型： 成肌细胞。 |
形态
Morphology |
成肌细胞 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Yaffe D, Saxel O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270: 725-727, 1977. PubMed: 563524Blau HM, et al. Plasticity of the differentiated state. Science 230: 758-766, 1985. PubMed: 2414846Katagiri T, et al. Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]. J. Cell Biol. 127: 1755-1766, 1994. PubMed: 7798324Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336Kessler PD, et al. Gene delivery to skeletal muscle results in sustanined expression and systemic delivery of a therapeutic protein. Proc. Natl. Acad. Sci. USA 93: 14082-14087, 1996. PubMed: 8943064Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936 |
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