缁嗚優鍩瑰吇鍩 >> 缁嗚優鏍
Beta-TC-6灏忛紶鑳板矝绱犵槫鑳板矝尾缁嗚優(2019骞存柊寮曡繘)
浜у搧浠嬬粛
| 鐩綍鍙饵br/>Serial |
SCSP-5061 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101MOUSCSP5061 |
鎻忚堪
Description |
璇ョ粏鑳炴潵婧愪簬鍦ㄨ浆鍩哄洜灏忛紶涓敓闀跨殑鑳拌吅鑲跨槫(鑳板矝绱犵槫)銆傝繖绉嶅皬榧犳惡甯︾敱澶ч紶鑳板矝绱營I鍩哄洜鍚姩瀛愭帶鍒剁殑SV40鏃╂湡鍖哄煙缁勬垚鐨勫亣鍩哄洜缁撴瀯銆傛嵁鎶ラ亾锛岃缁嗚優鍚湁涓板瘜鐨勮儼宀涚礌鍜屽皯閲忕殑鑳伴珮琛€绯栫礌鍜岀敓闀挎姂绱狅紝瀹冧滑鍝嶅簲钁¤悇绯栧垎娉岃儼宀涚礌銆侟br/>缁嗚優璐ㄦ帶缁撴灉锛欬br/>1.鏀師浣撴娴嬬粨鏋滐細姣忎釜鎵规鍧囬€氳繃鏈簱鏀師浣撴娴嬶紝缁撴灉涓洪槾鎬с€侟br/>2.STR閴村畾缁撴灉锛欬br/>鈶犺鏍粏鑳濪NA杩涜灏忛紶缁嗚優STR鍒嗗瀷缁撴灉鏄剧ず锛屾墿澧炲悗鍥捐氨娓呮櫚锛屽垎鍨嬬粨鏋滆壇濂斤細1-1锛?6锛?-2锛?6, 17锛?-1锛?6锛?-2锛?4锛?-2锛?2.3锛?-5锛?4, 15锛?-4锛?7, 18, 19锛?-7锛?2, 13锛?-1锛?5.2锛?-1锛?3锛?1-2锛?7锛?2-1锛?6锛?3-1锛?5.2, 16.2锛?5-3锛?2.3锛?7-2锛?4, 16锛?8-3锛?7, 18锛?9-2锛?4锛沊-1锛?4銆侟br/>鈶¤鏍粏鑳炵‘涓哄皬榧犵粏鑳烇紝娌℃湁浜烘簮缁嗚優姹℃煋銆侟/span> |
鍔ㄧ墿绉嶅埆
Organism |
灏忛紶锛孲V40澶鎶楀師杞熀鍥犲皬榧燑/span> |
鎬у埆
Gender |
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缁勭粐鏉ユ簮
Tissue and Cell Type |
鑳拌吅 |
| 褰㈡€?br/>Morphology |
涓婄毊鏍凤紝璐村鐢熼暱 |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
灏忛紶鑳板矝绱犵槫鑳板矝尾缁嗚優Beta-TC-6瀹屽叏鍩瑰吇娑查厤鏂?100 ml)锛欬br/>DMEM (Gibco, 11960-044) 83 ml
Glutamax (Gibco, 35050-061) 1 ml
Sodium Pyruvate (Gibco, 11360-070) 1 ml
FBS (Gibco) 15 ml
鐢熺墿瀹夊叏绛夌骇锛欱SL-2
鍙傝€冧紶浠ｆ瘮渚嬶細1:4
鍙傝€冧紶浠ｅ懆鏈燂細3-5澶?br/>鍙傝€冩崲娑查鐜囷細姣忓懆3娆狘br/>鍐诲瓨娑查厤鏂癸細瀹屽叏鍩瑰吇娑?5%锛孌MSO 5%
姘旂浉锛氱┖姘?5%锛屼簩姘у寲纰?%锛?7鈩冨煿鍏汇€侟br/>銆愭敞鎰忎簨椤广€慄br/>Beta-TC-6缁嗚優鐢熼暱缂撴參锛岃繖浜涚粏鑳炰粠鍐峰喕淇濆瓨涓仮澶嶉渶瑕佸嚑澶╃殑鏃堕棿銆傚紑濮嬫椂閫氬父鍏锋湁灏戦噺鍙瓨娲荤殑婕傛诞缁嗚優锛屽叾鏈€缁堜細褰㈡垚绨囧苟绮橀檮鍦ㄥ煿鍏荤摱搴曢儴锛屽畠浠垚绨囧湴杩炴帴骞跺舰鎴愬爢绉粏鑳炵殑宀涘笨銆傜粏鑳炰笉浼氬畬鍏?***铻嶅悎锛屽煿鍏荤墿涓婁細绮橀檮鍙瓨娲荤殑婕傛诞缁嗚優锛屽彲閫氳繃娓╁拰绂诲績淇濈暀銆傚湪娣诲姞缁嗚優鍓嶏紝鍩瑰吇鍩鸿嚦灏戣鎻愬墠15-30鍒嗛挓骞宠 娓╁害鍜孭H銆侟/span> |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Laurance ME, et al. Glucose responsive insulin secreting beta-cell lines and method for producing same. US Patent 5,773,255 dated Jun 30 1998 Poitout V, et al. Morphological and functional characterization of beta TC-6 cells--an insulin-secreting cell line derived from transgenic mice. Diabetes 44: 306-313, 1995. PubMed: 7533732 Poitout V, et al. Insulin-secreting cell lines: classification, characteristics and potential applications. Diabetes Metab. 22: 7-14, 1996. PubMed: 8697299 |
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鐩稿叧浜у搧
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鍦ㄧ嚎鐣欒█
