半岛bd体育手机客户端 介绍
目录号
Serial |
TCM34 |
标识符
Identifier |
CSTR:19375.09.3101MOUTCM34 |
描述
Description |
这株细胞来源于转基因小鼠中生长的一个胰肿瘤(胰岛素瘤)。 这种小鼠携带了大鼠胰岛素II基因启动子调控的SV40早期基因的假基因结构。 细胞包含丰富的胰岛素和小量的胰高血糖素及生长抑素。 响应葡萄糖而分泌胰岛素[PubMed:7533732]。
在本库通过支原体检测。 |
动物种别
Organism |
小鼠 |
性别
Gender |
*** |
组织来源
Tissue and Cell Type |
胰；胰岛素瘤；β细胞 |
形态
Morphology |
上皮细胞样 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，85%；优质热灭活胎牛血清，15%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Laurance ME , et al. Glucose responsive insulin secreting beta-cell lines and method for producing same. US Patent 5,773,255 dated Jun 30 1998Poitout V , et al. Morphological and functional characterization of beta TC-6 cells--an insulin-secreting cell line derived from transgenic mice. Diabetes 44: 306-313, 1995. PubMed: 7533732Poitout V , et al. Insulin-secreting cell lines: classification, characteristics and potential applications. Diabetes Metab. 22: 7-14, 1996. PubMed: 8697299Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
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