细胞培养基 >> 细胞株
BALB/3T3 clone A31小鼠胚胎成纤维细胞
半岛bd体育手机客户端 介绍
目录号
Serial |
GNM 3 |
标识符
Identifier |
CSTR:19375.09.3101MOUGNM3 |
描述
Description |
BALB/3T3 clone A31 是 1968年S.A. Aaronson和 G.T. Todaro从裂解的14到17天的 BALB/c 小鼠胚胎中建立的几株细胞之一(BALB/3T12)。
细胞分裂对接触抑制特别敏感，生长需高倍数稀释，饱和浓度低，对癌基因DNA病毒SV40和小鼠肉瘤病毒高度易感。
检测发现鼠痘病毒阴性。
2012年新引进。通过支原体检测。 |
动物种别
Organism |
小鼠 |
性别
Gender |
*** |
组织来源
Tissue and Cell Type |
器官：胚胎品系：BALB/c细胞类型：成纤维细胞 |
形态
Morphology |
成纤维细胞 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
22708: Aaronson SA, Todaro GJ. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J. Cell. Physiol. 72: 141-148, 1968. PubMed: 430100626022: Todaro GJ, Aaronson SA. Properties of clonal lines of murine sarcoma virus transformed Balb-3T3 cells. Virology 38: 174-202, 1969. PubMed: 430652326023: Aaronson SA, Todaro GJ. Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science 162: 1024-1026, 1968. PubMed: 430164726024: Jainchill JL, Todaro GJ. Stimulation of cell growth in vitro by serum with and without growth factor. Relation to contact inhibition and viral transformation. Exp. Cell Res. 59: 137-146, 1970. PubMed: 419442932554: Thompson SA, et al. COOH-terminal extended recombinant amphiregulin with bioactivity comparable with naturally derived growth factor. J. Biol. Chem. 271: 17927-17931, 1996. PubMed: 866353532907: Anderson MT, et al. Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins. Proc. Natl. Acad. Sci. USA 93: 8508-8511, 1996. PubMed: 871090092346: Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002.92389: Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999. |
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