半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-5012 |
标识符
Identifier |
CSTR:19375.09.3101MOUSCSP5012 |
描述
Description |
此细胞株于1962年由G. Todaro 和 H. Green从分解的Swiss小鼠胚胎中建立。其生长特性为接触抑制型，长满的单层细胞量是40000个/cm2，饱和密度可以达到约50000个/cm2。该细胞在玻璃表面上可能长不好，需要长在塑料培养瓶中。检测表明鼠痘病毒(ectromelia virus，ECTV)阴性。
每个批次均通过本库支原体检测，结果为阴性。
STR鉴定结果：
①该株细胞DNA进行小鼠细胞STR分型结果显示，扩增后图谱清晰，分型结果良好：1-1：10,15；1-2:13；2-1:9；3-2：14；4-2:19.3；5-5:13,15；6-4:15.2；6-7：12,15；7-1:25.2,29；8-1:15,16；11-2:15；12-1:19；13-1：15.1；15-3:20.3；17-2:12,15；18-3:17,21；19-2:12；X-1:26。
②该株细胞确为小鼠细胞，没有人源细胞污染。 |
动物种别
Organism |
小鼠 |
性别
Gender |
|
组织来源
Tissue and Cell Type |
胚胎 |
形态
Morphology |
成纤维细胞样，贴壁生长 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
小鼠胚胎成纤维细胞3T3-Swiss albino完全培养液配方(100 ml)：
DMEM (Invitrogen, 11960-044) 88 ml
新生牛血清(Gibco)灭活后使用 10 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml
(备注：细胞不能过密，请于细胞融合度80%时或之前传代)
不能使用胎牛血清FBS培养该细胞！ |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244Bennicelli JL, et al. Mechanism for transcriptional gain of function resulting from chromosomal translocation in alveolar rhabdomyosarcoma. Proc. Natl. Acad. Sci. USA 93: 5455-5459, 1996. PubMed: 8643596Vogt M, Dulbecco R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology 16: 41-51, 1962. PubMed: 13926482Todaro GJ, et al. Antigenic and cultural properties of cells doubly transformed by polyoma virus and SV40. Virology 27: 179-185, 1965. PubMed: 4284655Todaro GJ, et al. Transformation of properties of an established cell line by SV40 and polyoma virus. Proc. Natl. Acad. Sci. USA 51: 66-73, 1964. PubMed: 14104605Fang X, et al. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. J. Biol. Chem. 272: 13683-13689, 1997. PubMed: 9153219Chen ST, et al. Generation of packaging cell lines for pseudotyped retroviral vectors of the G protein of vesicular stomatitiis virus by using a modified tetracycline inducible system. Proc. Natl. Acad. Sci. USA 93: 10057-10062, 1996. PubMed: 8816750Campbell M, et al. The simian foamy virus type 1 transcriptional transactivator (Tas) binds and activates an enhancer element in the gag gene. J. Virol. 70: 6847-6855, 1996. PubMed: 8794326Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936 |
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