半岛bd体育手机客户端 介绍
目录号
Serial |
GNM25 |
标识符
Identifier |
CSTR:19375.09.3101MOUGNM25 |
描述
Description |
L1是通过克隆分离得到的3T3 (swiss小白鼠)的连续亚株。 当细胞从快速分裂到长满且接触抑制时，该细胞经过前脂肪向脂肪样逆转。 培液中，高血清含量可以促进脂肪积累。
在本库通过支原体检测。 |
动物种别
Organism |
小鼠 |
性别
Gender |
未知 |
组织来源
Tissue and Cell Type |
胚胎 |
形态
Morphology |
成纤维细胞 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
886: Green H, Meuth M. An established pre-adipose cell line and its differentiation in culture. Cell 3: 127-133, 1974. PubMed: 44260903491: Green H. Triglyceride-accumulating clonal cell line. US Patent 4,003,789 dated Jan 18 197732373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 870926032455: Scherer PE, et al. Identification, sequence, and expression of caveolin-2 defines a caveolin gene family. Proc. Natl. Acad. Sci. USA 93: 131-135, 1996. PubMed: 855259032787: Kallen CB, Lazar MA. Antidiabetic thiazolidinediones inhibit leptin (ob) gene expression in 3T3-L1 adipocytes. Proc. Natl. Acad. Sci. USA 93: 5793-5796, 1996. PubMed: 8650171 |
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