半岛bd体育手机客户端 介绍
目录号
Serial |
TCHu164 |
标识符
Identifier |
CSTR:19375.09.3101HUMTCHu164 |
描述
Description |
超微结构有少许微绒毛，连接复合物，形态完整的高尔基体，内质网多为光滑型，脂滴，没有病毒棵粒。
在本库通过支原体检测。
在本库通过STR检测。 |
动物种别
Organism |
人 |
性别
Gender |
女 |
组织来源
Tissue and Cell Type |
肾，肋膜渗出液 |
形态
Morphology |
圆形悬浮 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
McCOY's 5A(SIGMA,货号M4892，添加NaHCO3 2.2g/L)，85%；优质胎牛血清，15%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Fogh J, editor. Human tumor cells in vitro. New York: Plenum Press; 1975, pp. 115-159.Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080Fogh J . Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047Orkin SH , et al. Development of homozygosity for chromosome 11p markers in Wilms' tumour. Nature 309: 172-174, 1984. PubMed: 6325937Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
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