缁嗚優鍩瑰吇鍩 >> 缁嗚優鏍
SH-SY5Y浜虹缁忔瘝缁嗚優鐦?2016骞存柊寮曡繘)
浜у搧浠嬬粛
| 鐩綍鍙饵br/>Serial |
SCSP-5014 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101HUMSCSP5014 |
鎻忚堪
Description |
浜虹缁忔瘝缁嗚優鐦ゅ缓绯讳簬1970骞?鏄缁忔瘝缁嗚優鐦K-N-SH缁嗚優绯荤粡涓夋鍏嬮殕鍚庣殑浜氱郴(SK-N-SH鈫扴H-SY鈫扴H-SY5鈫扴H-SY5Y)銆傛嵁鎶ラ亾SH-SY5Y缁嗚優鐨勯ケ鍜屽瘑搴﹀ぇ浜?000000涓?cm2,缁嗚優琛ㄨ揪涓瓑鐨勫宸磋兒尾缇熷寲閰舵椿鎬с€傛瘡涓壒娆″潎閫氳繃鏈簱鏀師浣撴娴嬶紝缁撴灉涓洪槾鎬с€傜粡鏈簱STR妫€娴嬬粨鏋滄棤璇€係TR缁撴灉濡備笅锛欰melogenin: X锛汣SF1PO: 11锛汥13S317: 11锛汥16S539: 8,13锛汥5S818: 12锛汥7S820: 7,10锛汿HO1: 7,10锛汿POX: 8,11锛泇WA: 14,18 |
鍔ㄧ墿绉嶅埆
Organism |
浜裹/span> |
鎬у埆
Gender |
濂虫€?/span> |
缁勭粐鏉ユ簮
Tissue and Cell Type |
楠ㄩ珦 |
| 褰㈡€?br/>Morphology |
涓婄毊鏍凤紝璐村缁嗚優涓庢偓娴粏鑳炲悓鏃跺瓨鍦?/span> |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
浜虹缁忔瘝缁嗚優鐦ょ粏鑳濻H-SY5Y瀹屽叏鍩瑰吇娑查厤鏂?100 ml):
MEM(invitrogen 11090081)43.5ml
F12 (invitrogen 11765054) 43.5ml
FBS (Gibco) 10 ml
Gluta-max(invitrogen 35050061) 1 ml
Sodium pyruvate(invitrogen 11360070) 1 ml
NEAA (Invitrogen, 11140050) 1 ml
鍙傝€冧紶浠ｅ懆鏈燂細6-8澶?br/>鍙傝€冧紶浠ｆ瘮渚嬶細1:3-1:5
鍙傝€冩崲娑查鐜囷細3-4澶?br/>鍐诲瓨娑诧細瀹屽叏鍩瑰吇娑?5%锛 DMSO 5%
姘旂浉锛氱┖姘旓紝95%锛涗簩姘у寲纰筹紝5%銆傛俯搴︼細37鎽勬皬搴︺€侟br/>娉ㄦ剰浜嬮」锛氳缁嗚優鍩瑰吇鏃惰创澹佺粏鑳炰笌鎮诞缁嗚優鍚屾椂瀛樺湪锛屽潎涓烘甯稿舰鎬佺殑娲荤粏鑳烇紝鍩瑰吇杩囩▼涓彲鑳戒細閬囧埌浠ヤ笅鎯呭喌锛欬br/>(1)濡傛灉鍦ㄥ煿鍏昏繃绋嬩腑鍙戠幇缁嗚優鍩烘湰涓婂憟鐜拌创澹佺姸鎬侊紝寰堝皯鏈夋偓娴殑娲荤粏鑳烇紝杩欐槸姝ｅ父鎯呭喌锛屾垜浠缓璁偍蹇界暐鎮诞鐨勭粏鑳烇紝鎸夌収璐村缁嗚優鐨勫煿鍏绘柟妗堣繘琛屽煿鍏诲嵆鍙€侟br/>(2)濡傛灉鍩瑰吇杩囩▼涓彂鐜版偓娴殑缁嗚優鏁伴噺姣旇緝澶氾紝鎴栬€呯粏鑳炶櫧鐒朵负璐村鐘舵€佷絾瀹规槗鑱氬洟锛屽苟涓旂敓闀跨紦鎱紝鍒欓渶瑕佸湪鎹㈡恫鍜屼紶浠ｆ椂鏀堕泦杩欎簺鎮诞缁嗚優缁х画鍩瑰吇锛屽苟涓斿敖蹇洿鎹㈤珮璐ㄩ噺鐨勮娓呬互淇冭繘缁嗚優璐村鍜岀敓闀裤€侟/span> |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. PubMed: 6137586Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. PubMed: 6137586 |
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