浜у搧浠嬬粛
| 鐩綍鍙饵br/>Serial |
SCSP-5025 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101HUMSCSP5025 |
鎻忚堪
Description |
涓€浣?4宀佺櫧浜虹敺鎬х殑姝ｅ父鍓嶅垪鑵虹粍缁囧垏鐗囩殑鍛ㄥ洿鍖哄煙鐨勪笂鐨粏鑳炵敤鍗曟嫹璐濈殑浜轰钩澶寸槫鐥呮瘨18(HPV-18)杩涜杞寲锛屽缓绔嬩簡RWPE-1銆傛嵁鎶ラ亾锛孯WPE-1缁嗚優鍦ㄤ笁缁碝atrigel鍩瑰吇鏃讹紝鍦ㄩ泟婵€绱犲埡婵€涓嬶紝褰㈡垚鑵鸿優(acini)骞跺悜鍩瑰吇鍩轰腑鍒嗘硨PSA銆傚綋涓嶮atrigel鎴栧熀璐ㄧ粏鑳炴贩鍚堟敞灏勯泟鎬ц８榧犳椂锛孯WPE-1缁嗚優涔熻兘褰㈡垚鑵鸿優骞朵骇鐢烶SA銆傛潵婧愪簬RWPE-1鐨勭粏鑳炵敤Kirstin榧犺倝鐦ょ梾姣?Ki-MuSV)杞煋Ki-ras鍩哄洜锛屽缓绔嬩簡鑳芥垚鐦ょ殑RWPE-2缁嗚優鍜 RWPE2-W99缁嗚優銆傚彟澶栵紝鐢∟-鐢查唶-N-纭濆熀鑴?MNU)澶勭悊RWPE-1锛屽缓绔嬩簡涓€绯诲垪妯℃嫙鍓嶅垪鑵虹檶杩涚▼涓笉鍚屾椂鏈熺殑鎴愮槫缁嗚優鏍€傚畠浠垎鍒槸WPE1-NA22, WPE1-NB14, WPE1-NB11鍜 WPE1-NB26缁嗚優銆傛嵁鎶ラ亾锛孯WPE-1缁嗚優缁忚繃妫€娴嬪悗鍙戠幇涔欒倽鐥呮瘨銆佷笝鑲濈梾姣掑拰浜哄厤鐤己闄风梾姣掗兘鍛堥槾鎬с€侾CR璇佸疄璇ョ粏鑳炵郴HPV鐥呮瘨DNA搴忓垪鍛堥槼鎬с€傛瘡涓壒娆″潎閫氳繃鏈簱鏀師浣撴娴嬶紝缁撴灉闃存€с€傚湪鎴戝簱閫氳繃STR閴村畾锛屾暟鎹笂94%鍚诲悎銆係TR缁撴灉濡備笅锛欴5S818: 12,15锛汥13S317: 8,14锛汥7S820: 10,11锛汥16S539: 9,11锛泇WA: 18锛汿HO1: 8,9.3锛汚melogenin: X,Y锛汿POX: 8,11锛汣SF1PO: 13 |
鍔ㄧ墿绉嶅埆
Organism |
浜猴紱鐧戒汉 |
鎬у埆
Gender |
鐢锋€э紝54宀?/span> |
缁勭粐鏉ユ簮
Tissue and Cell Type |
鍓嶅垪鑵裹/span> |
| 褰㈡€?br/>Morphology |
涓婄毊鏍凤紝璐村鐢熼暱 |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
1. 浜哄墠鍒楄吅缁嗚優RWPE-1鍩瑰吇娑查厤鏂癸細Keratinocyte-SFM (Invitrogen, 17005-042)
K-SFM(Invitrogen锛?7005-042)鍩瑰吇娑插寘鍚袱涓粍浠斤細鍩虹鍩瑰吇娑?鐡?璐у彿10724-011)+鐢熼暱鍥犲瓙2绠?璐у彿37000-015 )銆傛寜鐓-SFM鍩瑰吇娑茶鏄庝功锛岄渶棰濆娣诲姞Gentamicin Solution銆傚缓璁娇鐢℅entamicin/Amphotericin Solution 500X(Gibco, R-015-10)銆侟br/>娉ㄦ剰浜嬮」锛欬br/>a. 缁嗚優鍦ㄦ棤琛€娓呭煿鍏讳綋绯讳腑鍩瑰吇锛屽叾璐村鑳藉姏杈冨急锛屽缓璁紶浠ｅ悗闈欑疆24灏忔椂鍐嶈繘琛屽悗缁搷浣溿€侟br/>b. 缁嗚優瀵嗗害寤鸿淇濇寔鍦?涓囷綖 7涓嘽ells/cm2锛屾帴绉嶅瘑搴﹀缓璁湪2涓囷綖4涓囨椿缁嗚優/cm2銆侟br/>c. 浣跨敤0.05%鑳伴叾娑堝寲缁嗚優銆傚悓鏃剁敱浜嶬-sfm涓烘棤琛€娓呭煿鍏绘恫鏃犳硶缁堟鑳伴叾娑堝寲锛屾墍浠ユ秷鍖栧畬鎴愬悗瑕侀€氳繃娣诲姞2%FBS(鐢―-PBS绋€閲?鎴栫瓑浣撶Н鐨?.1%澶ц眴鑳拌泲鐧介叾鎶戝埗鍓傜粓姝㈡秷鍖栧苟绂诲績鍘婚櫎鑳伴叾锛屽啀杩涜鍚庣画鎿嶄綔銆侟/span> |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Webber MM, Rhim JS. Immortalized and malignant human prostatic cell lines. US Patent 5,824,488 dated Oct 20 1998Bello D, et al. Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis 18: 1215-1223, 1997. PubMed: 9214605Webber MM, et al. Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. Carcinogenesis 18: 1225-1231, 1997. PubMed:9214606Okamoto M, et al. Interleukin-6 and epidermal growth factor promote anchorage-independent growth of immortalized human prostatic epithelial cells treated with N-methyl-N-nitrosourea. Prostate 35: 255-262, 1998. PubMed: 9609548Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed:9018337Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152Kremer R, et al. ras Activation of human prostate epithelial cells induces overexpression of parathyroid hormone-related peptide. Clin. Cancer Res. 3: 855-859, 1997. PubMed: 9815759Jacob K, et al. Osteonectin promotes prostate cancer cell migration and invasion: a possible mechanism for metastasis to bone. Cancer Res. 59: 4453-4457, 1999. PubMed: 10485497Achanzar WE, et al. Cadmium induces c-myc, p53, and c-jun expression in normal human prostate epithelial cells as a prelude to apoptosis. Toxicol. Appl. Pharmacol. 164: 291-300, 2000. PubMed: 10799339Achanzar WE, et al. Cadmium-induced malignant transformation of human prostate epithelial cells. Cancer Res. 61: 455-458, 2001. PubMed: 11212230Bello-DeOcampo D, et al. Laminin-1 and alpha6beta1 integrin regulate acinar morphogenesis of normal and malignant human prostate epithelial cells. Prostate 46: 142-153, 2001. PubMed:11170142Webber MM, et al. Human cell lines as an in vitro/in vivo model for prostate carcinogenesis and progression. Prostate 47: 1-13, 2001. PubMed: 11304724Epithelial cells from a histologically normal adult human prostate were isolated and subsequently transfected with a plasmid carrying one copy of the human papillomavirus 18 (HPV-18) genome to establish the RWPE-1 (ATCC CRL-11609) cell line.Quader ST, et al. Evaluation of the chemopreventive potential of retinoids using a novel in vitro human prostate carcinogenesis model. Mutat. Res. 496: 153-161, 2001. PubMed: 11551491upregulated upon exposure to androgenBello D, et al. Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis 18: 1215-1223, 1997. PubMed: 9214605 |
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鐩稿叧浜у搧
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鍦ㄧ嚎鐣欒█
