半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-5071 |
标识符
Identifier |
CSTR:19375.09.3101HUMSCSP5071 |
描述
Description |
此细胞株源自一位45岁患有大细胞肺癌的白人男性的淋巴结。该细胞缺乏产生粘液和鳞状分化的亚显微结构和生化证据。其表达的p53 mRNA与正常肺细胞的表达量相近，且易于检测。此外，没有表现出明显的结构DNA异常。该细胞角蛋白和波形蛋白表达阳性，神经丝三联体蛋白阴性。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X,Y；CSF1PO: 10；D13S317: 11；D16S539: 11；D5S818: 12；D7S820: 8,12；TH01: 9.3；TPOX: 8；vWA: 17,18。 |
动物种别
Organism |
白人 |
性别
Gender |
男性，45岁 |
组织来源
Tissue and Cell Type |
肺；来源于转移部位：淋巴结 |
形态
Morphology |
上皮样，贴壁生长 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
人大细胞肺癌细胞NCI-H661 [H661] 完全培养液配方(100 ml)：
RPMI 1640 Medium (Invitrogen, 11875-093)88 ml
FBS (Gibco)10 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml
参考传代比例：1：3
参考传代周期：3～5天
参考换液频率：每周2次
冻存液配方：完全培养液95%，DMSO 5% |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Lieblich JM, et al. Ectopic and eutopic secretion of chorionic gonadotropin and its subunits in vitro: Comparison of clonal strains from carcinomas of lung and placenta. J. Natl. Cancer Inst. 56: 911-917, 1976. PubMed: 62839Rabson AS, et al. Production of human chorionic gonadotropin in vitro by a cell line derived from a carcinoma of the lung. J. Natl. Cancer Inst. 50: 669-674, 1973. PubMed: 4708151Dahiya R, et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993. PubMed: 8443822Tashjian AH Jr., et al. Subunits of human chorionic gonadotropin: unbalanced synthesis and secretion by clonal cell strains derived from a bronchogenic carcinoma. Proc. Natl. Acad. Sci. USA 70: 1419-1422, 1973. PubMed: 4514312Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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