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细胞培养基 >> 细胞株
NCI-H661 [H661]人大细胞肺癌细胞(2021年新引进)
NCI-H661 [H661]人大细胞肺癌细胞(2021年新引进)图片
半岛bd体育手机客户端 介绍
目录号
Serial
SCSP-5071
标识符
Identifier
CSTR:19375.09.3101HUMSCSP5071
描述
Description
此细胞株源自一位45岁患有大细胞肺癌的白人男性的淋巴结。该细胞缺乏产生粘液和鳞状分化的亚显微结构和生化证据。其表达的p53 mRNA与正常肺细胞的表达量相近,且易于检测。此外,没有表现出明显的结构DNA异常。该细胞角蛋白和波形蛋白表达阳性,神经丝三联体蛋白阴性。每个批次均通过本库支原体检测,结果为阴性。经本库STR检测无误。STR鉴定结果为:Amelogenin: X,Y;CSF1PO: 10;D13S317: 11;D16S539: 11;D5S818: 12;D7S820: 8,12;TH01: 9.3;TPOX: 8;vWA: 17,18。
动物种别
Organism
白人
性别
Gender
男性,45岁
组织来源
Tissue and Cell Type
肺;来源于转移部位:淋巴结
形态
Morphology
上皮样,贴壁生长
培养基和添加剂
Complete Growth Medium and Culture Conditions
人大细胞肺癌细胞NCI-H661 [H661] 完全培养液配方(100 ml):
RPMI 1640 Medium (Invitrogen, 11875-093)88 ml
FBS (Gibco)10 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml
参考传代比例:1:3
参考传代周期:3~5天
参考换液频率:每周2次
冻存液配方:完全培养液95%,DMSO 5%
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCE Lieblich JM, et al. Ectopic and eutopic secretion of chorionic gonadotropin and its subunits in vitro: Comparison of clonal strains from carcinomas of lung and placenta. J. Natl. Cancer Inst. 56: 911-917, 1976. PubMed: 62839Rabson AS, et al. Production of human chorionic gonadotropin in vitro by a cell line derived from a carcinoma of the lung. J. Natl. Cancer Inst. 50: 669-674, 1973. PubMed: 4708151Dahiya R, et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993. PubMed: 8443822Tashjian AH Jr., et al. Subunits of human chorionic gonadotropin: unbalanced synthesis and secretion by clonal cell strains derived from a bronchogenic carcinoma. Proc. Natl. Acad. Sci. USA 70: 1419-1422, 1973. PubMed: 4514312Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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