细胞培养基 >> 细胞株
NCI-H460 [H460]人大细胞肺癌细胞(2016年新引进)
半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-584 |
标识符
Identifier |
CSTR:19375.09.3101HUMSCSP584 |
描述
Description |
人大细胞肺癌细胞NCI-H460 [H460]由A.F. Gazdar于1982年建立，来源于一位大细胞肺癌患者的胸腔积液。该细胞与正常肺组织p53 mRNA的表达水平相近，角蛋白(keratin)和波形蛋白(vimentin)表达阳性，神经丝三联体蛋白(neurofilament triplet protein)表达阴性。该细胞DNA总体结构未现异常。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X,Y; CSF1PO: 11,12; D13S317: 13; D16S539: 9; D5S818: 9,10; D7S820: 9,12; THO1: 9.3; TPOX: 8; vWA: 17 |
动物种别
Organism |
人 |
性别
Gender |
男 |
组织来源
Tissue and Cell Type |
肺；胸腔积液 |
形态
Morphology |
上皮样 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
人肺癌细胞NCI-H460 [H460]完全培养液配方(100 ml)：
RPMI 1640 Medium (Invitrogen, 11875093)88 ml
FBS (Gibco)10 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate (Invitrogen, 11360070) 1 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 |
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