半岛bd体育手机客户端 介绍
目录号
Serial |
TCHu151 |
标识符
Identifier |
CSTR:19375.09.3101HUMTCHu151 |
描述
Description |
1981年从一位开始化疗之前的患者的肿瘤组织中分离建株。 超微结构研究表明细胞质中有Clara细胞的特征结构 细胞表达主要的肺表面结合蛋白SP-A的蛋白和RNA。 不表达SP-B和SP-C。 他们在软琼脂中的克隆形成效率为0.83%。
在本库通过支原体检测。
在本库通过STR检测。 |
动物种别
Organism |
人 |
性别
Gender |
男 |
组织来源
Tissue and Cell Type |
细支气管及肺泡癌;非小细胞肺癌;肺;细支气管;肺泡 |
形态
Morphology |
上皮细胞样 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
RPMI 1640培养基(GIBCO by life technologies ,货号C11875500BT)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Tomlinson GE et al. Chaacterization of a breast cancer cell line derived from a germ-line BRCA1 mutation. Cancer Res. 58:3237-3242, 1998 PubMed: 98363228Gazdar AF et al. Charaterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998 PubMed: 99049517Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
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