半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-5075 |
标识符
Identifier |
CSTR:19375.09.3101HUMSCSP5075 |
描述
Description |
细胞表达相对大量的c-myc mRNA，但c-myc DNA序列不扩增。
表达c-myb, v-fms, Ha-ras, Ki-ras, N-ras, c-raf 1 mRNAs。该细胞高表达四种生化标记物：神经元特异性烯醇酶(neuron specific enolase)、肌酸激酶的脑同工酶(brain isoenzyme of creatine kinase)、L-DOPA脱羧酶(L-DOPA decarboxylase)和类蛙皮素免疫活性(bombesin-like immunoreactivity)。
每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X, X；CSF1PO: 11,12；D13S317: 11,12；D16S539: 11,11；D5S818: 12,12；D7S820: 9,10；TH01: 6,9.3；TPOX: 8,11；vWA: 14,16。 |
动物种别
Organism |
白人 |
性别
Gender |
男性，59岁 |
组织来源
Tissue and Cell Type |
肺; 来源于转移灶：骨髓 |
形态
Morphology |
悬浮，多个细胞聚团 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
人小细胞肺癌细胞 NCI-H146 [H146] 完全培养液 配方(100 ml)：
RPMI 1640 Medium (Invitrogen, 11875-093)88 ml
FBS (Gibco)10 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml
参考传代比例：1:2-1:3
参考传代周期：4-6天
参考换液频率：每2-3天换1次液
冻存液配方：完全培养液95%，DMSO 5%
【注意事项：】该细胞传代时可以通过加入新鲜培养基稀释或者通过离心收集并将细胞重悬浮在新鲜培养基中。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893Lung Cancer 4: 155-161, 1988.The NCI-H146 cell line was derived by A.F. Gazdar and associates in 1979 from a bone marrow metastasis of a patient with small cell cancer of the lung. The bone marrow specimen was taken prior to therapy.The cells form transplantable tumors which histologically resemble tumor cells from the original biopsy specimen. |
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