缁嗚優鍩瑰吇鍩 >> 缁嗚優鏍
MV-4-11浜洪珦鎬у崟鏍哥粏鑳炵櫧琛€鐥呯粏鑳濣/div>
浜у搧浠嬬粛
| 鐩綍鍙饵br/>Serial |
SCSP-5031 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101HUMSCSP5031 |
鎻忚堪
Description |
MV-4-11缁嗚優绯荤敱Rovera璇鹃缁勫缓绔嬶紝鏉ユ簮浜庝竴鍚嶆偅鏈変汉鍙岃〃鍨嬮珦鎬у崟鏍哥粏鑳炵櫧琛€鐥?biphenotypic B myelomonocytic leukemia)鐨?0宀佺敺瀛╃殑澶栧懆琛€銆侷nterleukin (IL)-3鍙互鐙珛鍦版敮鎸佽缁嗚優闀挎湡鐢熼暱锛屼娇鐢?0%FBS鍩瑰吇鏃跺垯涓嶉渶瑕侀澶栨坊鍔營L-3銆備絾鏄紝鍦↖L-3鍜岀敓闀垮洜瀛怗ranulocyte/Macrophage Colony-Stimulating Factor (GM-CSF)鍧囧浜庝綆娴撳害鐨勬儏鍐典笅锛孖L-3浼氭姂鍒禡V-4-11缁嗚優鐨勫娈栥€傜敓闀垮洜瀛怗ranulocyte Colony Stimulating Factor(G-CSF)浼氬崗鍚孏M-CSF淇冭繘MV-4-11缁嗚優鐨勫娈栵紝鑰屽崟鐙殑G-CSF浼氱煭鏆傚埡婵€璇ョ粏鑳炵郴銆傛嵁鏂囩尞[PubMed: 3500218]鎶ラ亾锛岄棿鎺ュ厤鐤崸鍏夋硶妫€娴嬮珦鎬у崟鏍哥粏鑳炴姉鍘烠D15锛岃秴杩?6%鐨勮缁嗚優涓洪槼鎬э紝40锝?6%涓哄崟鏍哥粏鑳炴姉鍘烠D4闃虫€э紝4-11%涓哄崟鏍哥粏鑳炴姉鍘烠D10闃虫€с€傛瘡涓壒娆″潎閫氳繃鏈簱鏀師浣撴娴嬶紝缁撴灉涓洪槾鎬с€傛瘡涓壒娆″潎閫氳繃鏈簱鏀師浣撴娴嬶紝缁撴灉涓洪槾鎬с€傜粡鏈簱STR妫€娴嬫棤璇€係TR閴村畾缁撴灉涓猴細Amelogenin: X,Y锛汣SF1PO: 10,12锛汥13S317: 13锛汥16S539: 11,12锛汥5S818: 11,12锛汥7S820: 8,9锛汿HO1: 8,9.3锛汿POX: 8,11锛泇WA: 14,15 |
鍔ㄧ墿绉嶅埆
Organism |
浜裹/span> |
鎬у埆
Gender |
鐢锋€э紝10宀?/span> |
缁勭粐鏉ユ簮
Tissue and Cell Type |
澶栧懆琛€ |
| 褰㈡€?br/>Morphology |
鎴愭穻宸寸粏鑳炵姸锛屾偓娴敓闀军/span> |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
浜洪珦鎬у崟鏍哥粏鑳炵櫧琛€鐥呯粏鑳濵V-4-11瀹屽叏鍩瑰吇娑查厤鏂?100 ml)锛欬br/>IMDM (Invitrogen, 31980-030)90 ml
FBS (Gibco)10 ml
鍙傝€冧紶浠ｆ瘮渚嬶細缁嗚優鎺ョ瀵嗗害20涓囦釜/mL 锛屽煿鍏绘椂缁存寔缁嗚優瀵嗗害鍦?0涓?100涓囦釜/mL
鍙傝€冩崲娑查鐜囷細姣忓懆2娆狘br/>鍐诲瓨娑诧細瀹屽叏鍩瑰吇娑?5%锛孌MSO 5%
鎴栧喕瀛樻恫锛氬巶鍟嗭細ZENOAQ锛岃揣鍙凤細CellBanker2銆侟br/>姘旂浉锛氱┖姘旓紝95%锛涗簩姘у寲纰筹紝5%銆傛俯搴︼細37鎽勬皬搴︺€侟br/>銆愭敞鎰忎簨椤广€戯細
a) 璇ョ粏鑳炰负鎮诞缁嗚優锛屾牴鎹煿鍏荤粡楠屼互鍙婂鎴风殑鍙嶉锛屼紶浠ｆ椂浣跨敤銆愬崐鎹㈡恫娉曘€戝缁嗚優鐘舵€佽緝涓烘湁鍒╋紝鍥犳鎴戝簱寤鸿鎮ㄤ娇鐢ㄣ€愬崐鎹㈡恫娉曘€戣繘琛屼紶浠ｃ€傚悓鏃讹紝鎮ㄥ湪鏀跺埌缁嗚優鍚庯紝璇蜂笉瑕侀€氳繃绂诲績鐨勬柟寮忔敹闆嗙粏鑳烇紝鍙互鐩存帴鍚戝煿鍏荤摱涓坊鍔犵瓑浣撶Н鐨勬柊椴滃煿鍏绘恫锛岀劧鍚庡皢缁嗚優鍚规墦鍧囧寑鍚庣Щ鍏ヤ袱涓柊鐨凾25鍩瑰吇鐡朵腑缁х画鍩瑰吇鍗冲彲銆侟br/>b) 缁嗚優瀵硅娓呰川閲忚緝涓烘晱鎰燂紝鎴戝簱寤鸿鎮ㄤ娇鐢ㄨ繘鍙ｅぇ鍝佺墝浼樿川琛€娓呰繘琛屽煿鍏汇€侟br/>c) 璇ョ粏鑳炲缁嗚優瀵嗗害杈冧负鏁忔劅锛屽煿鍏汇€佷紶浠ｆ椂璇锋敞鎰忎繚鎸佺粏鑳炲瘑搴﹀湪鍚堥€傜殑鑼冨洿銆傜粏鑳炴帴绉嶅瘑搴?0涓囦釜/mL 锛屽煿鍏绘椂缁存寔缁嗚優瀵嗗害鍦?0涓?100涓囦釜/mL |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Lange B, et al. Growth factor requirements of childhood acute leukemia: establishment of GM-CSF-dependent cell lines. Blood 70: 192-199, 1987. PubMed: 3496132Santoli D, et al. Synergistic and antagonistic effects of recombinant human interleukin (IL) 3, IL-1 alpha, granulocyte and macrophage colony-stimulating factors (G-CSF and M-CSF) on the growth of GM-CSF-dependent leukemic cell lines. J. Immunol. 139: 3348-3354, 1987. PubMed: 3500218If the cells are maintained in a serum-free medium, it is necessary to add the following: 0.005 mg/ml transferrin, 0.005 mg/ml insulin, and 5 ng/ml GM-CSF. |
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鐩稿叧浜у搧
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