浜у搧浠嬬粛
| 鐩綍鍙饵br/>Serial |
SCSP-5040 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101HUMSCSP5040 |
鎻忚堪
Description |
杩欐牚缁嗚優鍙栬嚜14鍛ㄧ敺鎬ц儙鍎跨殑姝ｅ父鑲洪儴缁勭粐锛岀敱J.P.Jacobs浜?966骞村缓绯汇€傛甯告煋鑹蹭綋锛屽父鏌撹壊浣?6鏉℃鐜囦负70%銆傛煋鑹蹭綋澶氬€嶆鐜囦负3.6%銆傚喕瀛樻椂涓篜17浠ｃ€傜敤鎴锋敹鍒板悗鍙户缁紶浠?娆″乏鍙炽€傛瘡涓壒娆″潎閫氳繃鏈簱鏀師浣撴娴嬶紝缁撴灉涓洪槾鎬с€傜粡鏈簱STR妫€娴嬬粨鏋滄棤璇€係TR缁撴灉濡備笅锛欴5S818: 11,12锛汥13S317: 11,14锛汥7S820: 10,11锛汥16S539: 9,11锛泇WA: 15锛汿HO1: 8锛汚melogenin: X,Y锛汿POX: 6,8锛汣SF1PO: 11,12 |
鍔ㄧ墿绉嶅埆
Organism |
浜裹/span> |
鎬у埆
Gender |
鐢锋€э紝14鍛ㄨ儙鍎军/span> |
缁勭粐鏉ユ簮
Tissue and Cell Type |
鑲裹/span> |
| 褰㈡€?br/>Morphology |
鎴愮氦缁寸粏鑳炴牱锛岃创澹佺敓闀军/span> |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
浜鸿儦鑲虹粏鑳濵RC-5瀹屽叏鍩瑰吇娑查厤鏂?100 ml)锛欬br/>MEM (Gibco, 11090081) 87 ml
FBS (Gibco) 10 ml
Gluta-max(Gibco, 35050061) 1 ml
NEAA (Gibco, 11140050) 1 ml
Sodium pyruvate(Gibco, 11360070) 1 ml
姘旂浉锛氱┖姘?5%锛屼簩姘у寲纰?%锛?7鈩冨煿鍏汇€侟br/>鍙傝€冧紶浠ｅ懆鏈燂細5澶╁乏鍙颤br/>鍙傝€冧紶浠ｆ瘮渚嬶細1:2-1:4
鍙傝€冩崲娑查鐜囷細姣忓懆1-2娆狘br/>鍐诲瓨娑查厤鏂癸細瀹屽叏鍩瑰吇娑?5%锛孌MSO 5%
鐢熺墿瀹夊叏绛夌骇锛欱SL-1
銆愭敞鎰忎簨椤广€戯細
(1)璇ョ粏鑳炲DMSO鏁忔劅锛屾晠澶嶈嫃鏃跺簲绂诲績鍘诲鍐峰喕鍓傚悗鍐嶅煿鍏汇€侟br/>(2)璇ョ粏鑳炰负鏈夐檺缁嗚優绯伙紝鏈€澶氫紶浠ｈ嚦P23-P25(涓庡煿鍏绘柟寮忓拰琛€娓呰川閲忔湁鍏?锛屼箣鍚庣粏鑳炲娈栫紦鎱€€傚缓璁皯閲忔墿澧炲苟灏藉揩鐢ㄤ簬瀹為獙銆侟/span> |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Jacobs JP, et al. Characteristics of a human diploid cell designated MRC-5. Nature 227: 168-170, 1970. PubMed: 4316953.Proc. Symp. Human Diploid Cells. Zagreb, Yugoslavia: Yugoslav Acad. Sci. Arts; 1970.Holliday R, Tarrant GM. Altered enzymes in ageing human fibroblasts. Nature 238: 26-30, 1972. PubMed: 12635262Lewis CM, Tarrant GM. Error theory and ageing in human diploid fibroblasts. Nature 239: 316-318, 1972. PubMed: 12635218Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029Kaur A, et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002.Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999.Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02.Standard Test Method for Evaluation of Handwashing Formulation for Virus-Eliminating Activity using the Entire Hand. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2011-99.Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02. |
|
|
鐩稿叧浜у搧
|
鍦ㄧ嚎鐣欒█
