半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-5040 |
标识符
Identifier |
CSTR:19375.09.3101HUMSCSP5040 |
描述
Description |
这株细胞取自14周男性胎儿的正常肺部组织，由J.P.Jacobs于1966年建系。正常染色体，常染色体46条概率为70%。染色体多倍概率为3.6%。冻存时为P17代。用户收到后可继续传代5次左右。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测结果无误。STR结果如下：D5S818: 11,12；D13S317: 11,14；D7S820: 10,11；D16S539: 9,11；vWA: 15；THO1: 8；Amelogenin: X,Y；TPOX: 6,8；CSF1PO: 11,12 |
动物种别
Organism |
人 |
性别
Gender |
男性，14周胎儿 |
组织来源
Tissue and Cell Type |
肺 |
形态
Morphology |
成纤维细胞样，贴壁生长 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
人胚肺细胞MRC-5完全培养液配方(100 ml)：
MEM (Gibco, 11090081) 87 ml
FBS (Gibco) 10 ml
Gluta-max(Gibco, 35050061) 1 ml
NEAA (Gibco, 11140050) 1 ml
Sodium pyruvate(Gibco, 11360070) 1 ml
气相：空气95%，二氧化碳5%；37℃培养。
参考传代周期：5天左右
参考传代比例：1:2-1:4
参考换液频率：每周1-2次
冻存液配方：完全培养液95%，DMSO 5%
生物安全等级：BSL-1
【注意事项】：
(1)该细胞对DMSO敏感，故复苏时应离心去处冷冻剂后再培养。
(2)该细胞为有限细胞系，最多传代至P23-P25(与培养方式和血清质量有关)，之后细胞增殖缓慢。建议少量扩增并尽快用于实验。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Jacobs JP, et al. Characteristics of a human diploid cell designated MRC-5. Nature 227: 168-170, 1970. PubMed: 4316953.Proc. Symp. Human Diploid Cells. Zagreb, Yugoslavia: Yugoslav Acad. Sci. Arts; 1970.Holliday R, Tarrant GM. Altered enzymes in ageing human fibroblasts. Nature 238: 26-30, 1972. PubMed: 12635262Lewis CM, Tarrant GM. Error theory and ageing in human diploid fibroblasts. Nature 239: 316-318, 1972. PubMed: 12635218Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029Kaur A, et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002.Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999.Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02.Standard Test Method for Evaluation of Handwashing Formulation for Virus-Eliminating Activity using the Entire Hand. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2011-99.Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02. |
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