半岛bd体育手机客户端 介绍
目录号 Serial |
GNHu41 |
标识符 Identifier |
CSTR:19375.09.3101HUMGNHu41 |
描述 Description |
MRC-5细胞系来自14周龄男性胎儿的正常肺组织,该细胞老化前能传代42到46次群体倍增。 它是正常二倍体细胞系,46,XY核型。 模式染色体数为46,概率为70%。 在本库通过支原体检测。 在本库通过STR检测。 |
动物种别 Organism |
人 |
性别 Gender |
男 |
组织来源 Tissue and Cell Type |
肺 |
形态 Morphology |
成纤维细胞 |
培养基和添加剂 Complete Growth Medium and Culture Conditions |
MEM培养基(GIBCO,货号41500034,添加NaHCO3 1.5g/L,Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 |
供应限制 Permits and Restrictions |
A。仅供研究之用。 |
REFERENCE |
Jacobs JP , et al. Characteristics of a human diploid cell designated MRC-5. Nature 227: 168-170, 1970. PubMed: 4316953Proc. Symp. Human Diploid Cells. Zagreb, Yugoslavia: Yugoslav Acad. Sci. Arts; 1970, pp. 43-55.Holliday R , Tarrant GM . Altered enzymes in ageing human fibroblasts. Nature 238: 26-30, 1972. PubMed: 12635262Lewis CM , Tarrant GM . Error theory and ageing in human diploid fibroblasts. Nature 239: 316-318, 1972. PubMed: 12635218Landers JE , et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029Kaur A , et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893MOLECULAR THERAPY 11(4):531,2005 |
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