浜у搧浠嬬粛
| 鐩綍鍙饵br/>Serial |
TCHu167 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101HUMTCHu167 |
鎻忚堪
Description |
杩欐牚缁嗚優鏄疕OS缁?.01mcg/ml MNNG(涓€绉嶈嚧鐧岀殑浜氱鑳?杞寲鑰屾潵銆 杩欐牚缁嗚優楗卞拰娴撳害楂橈紝鍦ㄨ蒋鐞艰剛涓殑鍏嬮殕褰㈡垚鐜囬珮锛屽苟鑳藉湪瑁搁紶涓垚鐦ゃ€侟br/>鍦ㄦ湰搴撻€氳繃鏀師浣撴娴嬨€侟br/>鍦ㄦ湰搴撻€氳繃STR妫€娴嬨€侟/span> |
鍔ㄧ墿绉嶅埆
Organism |
浜裹/span> |
鎬у埆
Gender |
濂颤/span> |
缁勭粐鏉ユ簮
Tissue and Cell Type |
楠紱楠ㄨ倝鐦｜/span> |
| 褰㈡€?br/>Morphology |
*** |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
MEM鍩瑰吇鍩?GIBCO,璐у彿41500034锛屾坊鍔燦aHCO3 1.5g/L锛孲odium Pyruvate 0.11g/L)锛?0%锛涗紭璐ㄨ儙鐗涜娓咃紝10%銆侟br/>姘旂浉锛氱┖姘旓紝95%锛涗簩姘у寲纰筹紝5%銆侟br/>娓╁害锛?7鎽勬皬搴︺€侟/span> |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Rhim JS , et al. Transformation of human cells in culture by N-methyl-N'-nitro-N- nitrosoguanidine. Nature 256: 751-753, 1975. PubMed: 1057046Rhim JS , et al. Characterization of human cells transformed in vitro by N-methyl-N'- nitro-N-nitrosoguanidine. Int. J. Cancer 19: 505-510, 1977. PubMed: 265298Zhu X , et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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鐩稿叧浜у搧
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![MNNG/HOS Cl #5 [R-1059-D]浜洪鑲夌槫缁嗚優鍥剧墖](http://www.vuestras.com/data/pub/pic/nopic180_135.jpg)
鍦ㄧ嚎鐣欒█
