浜у搧浠嬬粛
| 鐩綍鍙饵br/>Serial |
TCHu 32 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101HUMTCHu32 |
鎻忚堪
Description |
LS 174T鏄疞S 180缁撹偁鑵虹檶缁嗚優鏍殑鑳拌泲鐧介叾鍖栧彉绉嶃€ 瀹冩瘮浜叉湰鏇存槗浼犱唬锛岃薄LS 180涓€鏍风敓鎴愬ぇ閲忕殑鐧岃儦鎶楀師(CEA)銆 鐢甸暅鐮旂┒琛ㄦ槑鏈変赴瀵岀殑寰笣鍜岀粏鑳炶川绮樻恫绱犳恫娉°€ 鐩磋偁鎶楀師3闃虫€с€ p53鎶楀師琛ㄨ揪闃存€э紝浣唌RNA琛ㄨ揪闃虫€с€ LS 174T缁嗚優瑙掕泲鐧芥煋鑹查槼鎬с€ 鐧屽熀鍥燾-myc, N-myc, H-ras, N-ras, Myb, 鍜 fos鐨勮〃杈惧憟闃虫€с€ 鐧屽熀鍥爇-ras鍜宻is鐨勮〃杈炬湭鍋氭娴嬨€侟br/>鍦ㄦ湰搴撻€氳繃鏀師浣撴娴嬨€侟br/>鍦ㄦ湰搴撻€氳繃STR妫€娴嬨€侟/span> |
鍔ㄧ墿绉嶅埆
Organism |
浜裹/span> |
鎬у埆
Gender |
濂颤/span> |
缁勭粐鏉ユ簮
Tissue and Cell Type |
鐩磋偁锛涚粨鐩磋偁鑵虹檶 |
| 褰㈡€?br/>Morphology |
涓婄毊缁嗚優 |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
DMEM鍩瑰吇鍩?GIBCO by life technologies ,璐у彿C11995500BT)锛?0%锛涗紭璐ㄨ儙鐗涜娓咃紝10%銆侟br/>姘旂浉锛氱┖姘旓紝95%锛涗簩姘у寲纰筹紝5%銆侟br/>娓╁害锛?7鎽勬皬搴︺€侟/span> |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Tom BH , et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976. PubMed: 1262041Jakstys MM , et al. Process of producing carcinoembryonic antigen. US Patent 4,228,236 dated Oct 14 1980Chen TR , et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615Gastl GA , et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874Tsao H , et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066Shibata D , et al. Genomic instability in repeated sequences is an early somatic event in colorectal tumorigenesis that persists after transformation. Nat. Genet. 6: 273-281, 1994. PubMed: 8012390Kutchera W , et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486 |
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鐩稿叧浜у搧
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