半岛bd体育手机客户端 介绍
目录号 Serial |
SCSP-514 |
标识符 Identifier |
CSTR:19375.09.3101HUMSCSP514 |
描述 Description |
人结直肠癌细胞LoVo构建于1971年,来源于一名56岁的白人男性结直肠癌患者转移到左锁骨位置的一个肿瘤结节。CSAp和结肠抗原3(colon antigen 3)表达阴性。肿瘤基因c-myc、K-ras、H-ras、 N-ras、Myb, sis和fos表达阳性,但未检测到 N-myc表达。该细胞表达肿瘤特定的核基质蛋白CC-3和CC-4。每个批次均通过本库支原体检测,结果阴性。经本库STR检测结果无误,STR结果如下:D5S818: 11,13;D13S317: 8,11;D7S820:9.3,10,11;D16S539: 9,12;vWA: 17,18;THO1: 9.3;Amelogenin: X,Y;TPOX: 8,9;CSF1PO: 10,11,13,14 |
动物种别 Organism |
人;白人 |
性别 Gender |
男性,56岁 |
组织来源 Tissue and Cell Type |
结肠;分离自转移灶:左锁骨上区域 |
形态 Morphology |
上皮样,贴壁生长 |
培养基和添加剂 Complete Growth Medium and Culture Conditions |
完全培养液配方(100 ml) Ham's F-12K (Kaighn's) Medium (Invitrogen, 21127-022) 90 ml FBS (Gibco) 10 ml 参考传代周期:2-3天 参考传代比例:1:3-1:5 参考换液频率:每周2-3次 冻存液配方:完全培养液95%,DMSO 5% 气相:空气,95%;二氧化碳,5%。温度:37摄氏度。 |
供应限制 Permits and Restrictions |
A。仅供研究之用。 |
REFERENCE |
Drewinko B, et al. Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line. J. Natl. Cancer Inst. 61: 75-83, 1978. PubMed: 276641Drewinko B, Yand LY. Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells. Exp. Cell Res. 101: 414-416, 1976. PubMed: 964319Drewinko B, et al. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res. 36: 467-475, 1976. PubMed: 1260746Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905Drewinko B, et al. Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives. Cancer Res. 39: 2630-2636, 1979. PubMed: 445465Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538 |
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