半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-5062 |
标识符
Identifier |
CSTR:19375.09.3101HUMSCSP5062 |
描述
Description |
人肝癌细胞系。该细胞系由Tanno H. 建立自裸鼠体外移植肿瘤。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：D5S818: 9,11；D13S317:11；D7S820: 10,11；D16S539: 9；vWA: 16,19；TH01: 7；Amelogenin: X；TPOX: 8,11；CSF1PO: 12。 |
动物种别
Organism |
人 |
性别
Gender |
男性，45岁 |
组织来源
Tissue and Cell Type |
肝 |
形态
Morphology |
上皮样，贴壁生长 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
人肝癌细胞Li-7完全培养液配方(100 ml)：
RPMI 1640 Medium (Invitrogen, 11875-093)88 ml
FBS (Gibco)10 ml
Glutamax(invitrogen 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (invitrogen 11360070) 1 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
生物安全等级：BSL-1
参考传代比例：1:3
参考传代周期：3-5天
参考换液频率：每周2次
冻存液配方：完全培养液90%，DMSO 10% |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Ferraris P, Chandra PK, Panigrahi R, Aboulnasr F, Chava S, Kurt R, Pawlotsky JM, Wilkens L, Osterlund P, Hartmann R, Balart LA, Wu T, Dash S. Cellular Mechanism for Impaired Hepatitis C Virus Clearance by Interferon Associated with IFNL3 Gene Polymorphisms Relates to Intrahepatic Interferon-λ Expression. Am. J. Pathol. 2016 186:938-51 PubMed ID: 26896692Sugai S, Yoshikawa T, Iwama T, Tsuchiya N, Ueda N, Fujinami N, Shimomura M, Zhang R, Kaneko S, Uemura Y, Nakatsura T. Hepatocellular carcinoma cell sensitivity to Vγ9Vδ2 T lymphocyte-mediated killing is increased by zoledronate. Int. J. Oncol. 2016 48:1794-804 PubMed ID: 26936487Ohata T, Yokoo H, Kamiyama T, Fukai M, Aiyama T, Hatanaka Y, Hatanaka K, Wakayama K, Orimo T, Kakisaka T, Kobayashi N, Matsuno Y, Taketomi A. Fatty acid-binding protein 5 function in hepatocellular carcinoma through induction of epithelial-mesenchymal transition. Cancer Med 2017 6:1049-1061 PubMed ID: 28374947Fujisaka Y, Iwata T, Tamai K, Nakamura M, Mochizuki M, Shibuya R, Yamaguchi K, Shimosegawa T, Satoh K. Long non-coding RNA HOTAIR up-regulates chemokine (C-C motif) ligand 2 and promotes proliferation of macrophages and myeloid-derived suppressor cells in hepatocellular carcinoma cell lines. Oncol Lett 2018 PubMed ID: 29387231 |
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