半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-5054 |
标识符
Identifier |
CSTR:19375.09.3101HUMSCSP5054 |
描述
Description |
连续细胞株K-562来源于一位临终的53岁女性慢性髓性白血病患者的胸水。细胞被归入高度未分化的粒性白细胞类。Anderson等对其表面膜性质的研究表明，K-562是一株人类红白血病细胞。K-562母细胞是多能性，造血恶性细胞，它能自发分化成可识别的红血球祖细胞、粒性粒细胞、单核细胞等。Dimery等将多种K-562亚系分为3类(A,B,C)，此株细胞最接近B类，出现费城染色体，但频次较低，在15个分裂中期检测中未检测到。它是EBNA阴性的。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X；CSF1PO: 9,10；D13S317: 8；D16S539: 11,12；D5S818: 11,12；D7S820: 9,11；THO1: 9.3；TPOX: 8,9；vWA: 16。 |
动物种别
Organism |
人 |
性别
Gender |
女性，53岁 |
组织来源
Tissue and Cell Type |
骨髓 |
形态
Morphology |
成淋巴细胞样，悬浮生长 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
1.人慢性髓系白血病细胞K-562完全培养液配方(100 ml)：
IMDM (Invitrogen, 31980-030)90 ml
FBS (Gibco)10 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
参考传代比例：细胞接种密度10万个/mL，维持密度10万-100万个/mL.
参考换液频率：每周2次
冻存液配方：完全培养液95%，DMSO 5%
或冻存液：厂商：ZENOAQ，货号：CellBanker2。
2. 注意事项：
a) 该细胞为悬浮细胞，根据培养经验以及客户的反馈，传代时使用【半换液法】对细胞状态较为有利，因此我库建议您使用【半换液法】进行传代。同时，您在收到细胞后，请不要通过离心的方式收集细胞，可以直接向培养瓶中添加等体积的新鲜培养液，然后将细胞吹打均匀后移入两个新的T25培养瓶中继续培养即可。
b) 细胞对血清质量较为敏感，我库建议您使用进口大品牌优质血清进行培养。
c) 该细胞对细胞密度较为敏感，培养、传代时请注意保持细胞密度在合适的范围。细胞接种密度10万个/mL，维持密度10万-100万个/mL. |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765Ortaldo JR, et al. Specificity of natural cytotoxic reactivity of normal human lymphocytes against a myeloid leukemia cell line. J. Natl. Cancer Inst. 59: 77-82, 1977. PubMed: 69036Lozzio CB, Lozzio BB. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45: 321-334, 1975. PubMed: 163658Lozzio BB, Lozzio CB. Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Leuk. Res. 3: 363-370, 1979. PubMed: 95026Andersson LC, et al. K562--a human erythroleukemic cell line. Int. J. Cancer 23: 143-147, 1979. PubMed: 367973Lozzio BB, et al. A multipotential leukemia cell line (K-562) of human origin. Proc. Soc. Exp. Biol. Med. 166: 546-550, 1981. PubMed: 7194480Dimery IW, et al. Variation amongst K562 cell cultures. Exp. Hematol. 11: 601-610, 1983. PubMed: 6576909Chan YJ, et al. Two distinct upstream regulatory domains containing multicopy cellular transcription factor binding sites provide basal repression and inducible enhancer characteristics to the immediate-early IES (US3) promoter from human cytomegalovirus. J. Virol. 70: 5312-5328, 1996. PubMed: 8764042Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663Tiffany HL, et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J. Biol. Chem. 271: 12387-12393, 1996. PubMed: 8647842Chan YJ, et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. J. Virol. 70: 8590-8605, 1996. PubMed: 8970984Nauseel WM, et al. Effect of the R569W missense mutation on the biosynthesis of myeloperoxidase. J. Biol. Chem. 271: 9546-9549, 1996. PubMed: 8621627Grune T, et al. Degradation of oxidized proteins in K562 human hematopoietic cells by proteasome. J. Biol. Chem. 271: 15504-15509, 1996. PubMed: 8663134Jondal M, Pross H. Surface markers on human b and t lymphocytes. VI. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations. Int. J. Cancer 15: 596-605, 1975. PubMed: 806545West WH, et al. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells. J. Immunol. 118: 355-361, 1977. PubMed: 299761Pross HF, et al. Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. IX. The quantitation of natural killer cell activity. J. Clin. Immunol. 1: 51-63, 1981. PubMed: 7334070Chen TR. Modal karyotype of human leukemia cell line, K562 (ATCC CCL 243). Cancer Genet. Cytogenet. 17: 55-60, 1985. PubMed: 3857109Wu SQ, et al. Extensive amplification of bcr/abl fusion genes clustered on three marker chromosomes in human leukemic cell line K-562. Leukemia 9: 858-862, 1995. PubMed: 7769849 |
价格
Transmittal Fee |
1200 |
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