半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-5032 |
标识符
Identifier |
CSTR:19375.09.3101HUMSCSP5032 |
描述
Description |
HT-29细胞表达尿激酶受体，但未检测到纤溶酶原激活物活性[PubMed ID:8381394]。HT-29 细胞CD4表达阴性，但细胞表面表达半乳糖神经酰胺(一个HIV的可替代受体)。致癌基因c-myc、K-ras、H-ras、N-ras、Myb、sis、fos表达阳性，过量产生p53抗原，在p53基因的273密码子处有G->A突变导致Arg->His替换，未检测到致癌基因N-myc的表达。本库冻存时为P140左右。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测结果无误。STR结果如下：D5S818: 11,12；D13S317: 11,12；D7S820: 10；D16S539: 11,12；vWA: 17,19；THO1: 6,9；Amelogenin: X；TPOX: 8,9；CSF1PO: 11,12 |
动物种别
Organism |
人 |
性别
Gender |
女性，44岁 |
组织来源
Tissue and Cell Type |
结肠 |
形态
Morphology |
上皮样，贴壁生长 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
1. 人结肠癌细胞细胞HT-29完全培养液配方(100 ml)：
McCoy's 5a Medium (Invitrogen, 12330-031) 90 ml
FBS (Gibco) 10 ml
气相：空气95%，二氧化碳5%；37℃培养。
传代比例：1：3
传代周期：3-4天
换液频率：每周2次
冻存液：完全培养液95%，DMSO 5%
细胞状态：贴壁生长
2. 注意事项：
HT-29复苏后**周可能会有大量漂浮细胞，可更换新鲜培养液，同时将漂浮的细胞离心收集后加回到培养瓶中与贴壁细胞共同培养。新复苏的细胞会以补丁形态三维接触生长(即不是单层扁平生长)，待细胞分裂生长后将会变回上皮细胞形态单层扁平生长。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
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