半岛bd体育手机客户端 介绍
目录号
Serial |
TCHu170 |
标识符
Identifier |
CSTR:19375.09.3101HUMTCHu170 |
描述
Description |
这株细胞含有活化的N-ras癌基因。
在本库通过支原体检测。
在本库通过STR检测。 |
动物种别
Organism |
人 |
性别
Gender |
男 |
组织来源
Tissue and Cell Type |
结缔组织；纤维肉瘤 |
形态
Morphology |
上皮细胞 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
22147: Chen TR , et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet.Cytogenet. 10: 351-362, 1983. PubMed: 665261523071: Geiser AG , et al. Suppression of tumorigenicity in human cell hybrids derived from cell lines expressing differentactivated ras oncogenes. Cancer Res. 49: 1572-1577, 1989. PubMed: 264728923393: Rasheed S , et al. Characterization of a newly derived human sarcoma cell line (HT-1080). Cancer 33: 1027-1033,1974. PubMed: 413205325969: Adams RA , et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters,SB-2. Cancer Res. 28: 1121-1125, 1968. PubMed: 487271626035: Proc. Am. Assoc. Cancer Res. 8: 1, 1967.32289: Hu M , et al. Purification and characterization of human lung fibroblast motility-stimulating factor for human soft tissuesarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res. 57: 3577-3584, 1997. PubMed:927003132370: Iida A , et al. Inducible gene expression by retrovirus-mediated transfer of a modified tetracycline-regulated system. J.Virol. 70: 6054-6059, 1996. PubMed: 870922832531: Brenneman M , et al. Stimulation of intrachromosomal homologous recombination in human cells by electroporationwith site-specific endonucleases. Proc. Natl. Acad. Sci. USA 93: 3608-3612, 1996. PubMed: 862298333061: Seiffert D . Hydrolysis of platelet vitronectin by calpain. J. Biol. Chem. 271: 11170-11176, 1996. PubMed: 862666333152: Hocking AM , et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed:8702651 |
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