半岛bd体育手机客户端 介绍
目录号
Serial |
TCHu133 |
标识符
Identifier |
CSTR:19375.09.3101HUMTCHu133 |
描述
Description |
DNA fingerprinting证据表明，这株细胞和DLD-1来源于同一个人；但同工酶及细胞染色体组型分析仍存疑问。 细胞呈CSAp阴性(CSAp-)。 角蛋白免疫过氧化物酶染色阳性。
在本库通过支原体检测。
在本库通过STR检测。 |
动物种别
Organism |
人 |
性别
Gender |
男 |
组织来源
Tissue and Cell Type |
结直肠腺癌 |
形态
Morphology |
上皮细胞 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
RPMI 1640培养基(GIBCO by life technologies ,货号C11875500BT)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Chen TR , et al. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin. Cancer Genet. Cytogenet. 81: 103-108, 1995. PubMed: 762140422861: Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 333887423033: Dexter DL , et al. N,N-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells. Cancer Res. 39: 1020-1025, 1979. PubMed: 42774232266: Bender CM , et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 942606446971: Vermeulen SJ , et al. Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?. Cancer Genet. Cytogenet. 107: 76-79, 1998. PubMed: 9809040 |
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