半岛bd体育手机客户端 介绍
目录号
Serial |
TCHu 99 |
标识符
Identifier |
CSTR:19375.09.3101HUMTCHu99 |
描述
Description |
HCT116是1979年M.Brattain等从患结肠癌的男性病人中分离的三株恶性细胞之一。 在半固体琼脂糖培养基中形成克隆。 HCT116在无胸腺的裸鼠有致瘤性，形成上皮样的肿瘤。
在本库通过支原体检测。
在本库通过STR检测。 |
动物种别
Organism |
人 |
性别
Gender |
男 |
组织来源
Tissue and Cell Type |
结直肠癌 |
形态
Morphology |
上皮 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
McCOY's 5A(SIGMA,货号M4892，添加NaHCO3 2.2g/L)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Schroy PC , et al. Detection of p21ras mutations in colorectal adenomas and carcinomas by enzyme-linked immunosorbent assay. Cancer 76: 201-209, 1995. PubMed: 8625092Journal of Interferon & Cytokine Research 24:219,2004.Brattain MG , et al. Heterogeneity of malignant cells from a human colonic carcinoma. Cancer Res. 41: 1751-1756, 1981. PubMed: 7214343Sun L , et al. Autocrine transforming growth factor-beta 1 and beta 2 expression is increased by cell crowding and quiescence in colon carcinoma cells. Exp. Cell Res. 214: 215-224, 1994. PubMed: 8082724Santoro IM , Groden J . Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479Brattain MG , et al. Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. J. Natl. Cancer Inst. 69: 767-771, 1982. PubMed: 6956756Bender CM , et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064Landers JE , et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029Kutchera W , et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486Wang R , et al. Cellular adherence elicits ligand-independent activation of the Met cell-surface receptor. Proc. Natl. Acad. Sci. USA 93: 8425-8430, 1996. PubMed: 8710887MOLECULAR THERAPY 11(4):531,2005 |
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