半岛bd体育手机客户端 介绍
目录号
Serial |
TCHu153 |
标识符
Identifier |
CSTR:19375.09.3101HUMTCHu153 |
描述
Description |
这株细胞建于1994年三月。这株肺腺癌在EGFR酪氨酸激酶区域有一个获得性突变(E746-A750缺失)。 患者在25岁到26岁时每个月抽1包烟。 在诊断前12年不再抽烟。
在本库通过支原体检测。
在本库通过STR检测。 |
动物种别
Organism |
人 |
性别
Gender |
女 |
组织来源
Tissue and Cell Type |
肺腺癌 |
形态
Morphology |
上皮细胞样 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
RPMI 1640培养基(GIBCO by life technologies ,货号C11875500BT)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Girard L , et al. Genome-wide allelotyping of lung cancer identifies new regions of allelic loss, differences between small cell lung cancer and non-small cell lung cancer, and loci clustering. Cancer Res. 60: 4894-4906, 2000. PubMed: 10987304Burbee DG , et al. Epigenetic inactivation of RASSF1A in lung and breast cancers and malignant phenotype suppression. J. Natl. Cancer Inst. 93: 691-699, 2001. PubMed: 11333291Toyooka S , et al. Differential expression of FEZ1/LZTS1 gene in lung cancers and their cell cultures. Clin. Cancer Res. 8: PubMed: 12114433Virmani A , et al. Aberrant methylation of the cyclin D2 promoter in primary small cell, nonsmall cell lung and breast cancers. Int. J. Cancer 107: 341-345, 2003. PubMed: 14506731Liu CX , et al. LRP-DIT, a putative endocytic receptor gene, is frequently inactivated in non-small cell lung cancer cell lines. Cancer Res. 60: 1961-1967, 2000. PubMed: 10766186Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
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