浜у搧浠嬬粛
| 鐩綍鍙饵br/>Serial |
TCHu148 |
| 鏍囪瘑绗?br/>Identifier |
CSTR:19375.09.3101HUMTCHu148 |
鎻忚堪
Description |
杩欐牚缁嗚優1995骞?0鏈?3鏃ユ渶鍒濇潵婧愪簬鍘熷彂鎬у绠＄檶, 鐢ㄤ簡11.5涓湀寤烘牚銆侟br/>鑲跨槫鍒嗙被涓篢NM IIB鏈? 3绾с€侟br/>BRCA1鍒嗘瀽琛ㄦ槑杩欐牚缁嗚優鏄疊RCA1 5382C绐佸彉绾悎鐨? 鑰屾潵婧愪簬鍚屼竴鐥呬汉鐨勭被娣嬪反姣嶇粏鑳炵粏鑳炴牚鍦ㄨ繖涓獊鍙樹綅鐐逛笂鏄潅鍚堢殑銆 鍙︿袱涓搴垚鍛樹篃鏈夎繖涓獊鍙楙 涓€涓悓鍗靛弻鐢熷濡逛篃鎮ｆ湁涔宠吅鐧屻€侟br/>杩欐牚缁嗚優鏈変竴涓悗澶╃殑TP53绐佸彉, 鑰屽叾閲庣敓鍨嬬瓑浣嶅熀鍥犱涪澶胞 涓€涓狿TEN鍩哄洜鐨勫悗澶╃殑绾悎缂哄け, 浠ュ強澶氫釜涓庝钩鑵虹檶鍙戠梾鏈虹悊鐩稿叧鐨勪綅鐐逛笂鍙戠敓鐨勬潅鍚堢獊鍙樸€侟br/>杩欐牚缁嗚優Her2-neu鍜宲53琛ㄨ揪閮藉憟闃存€с€ HCC1937鐨勪笂鐨粏鑳炵壒寮傛€ф爣蹇椾笂鐨粏鑳炵硸铔?(EGP2)鍜岀粏鑳炶铔嬬櫧19閮藉憟闃虫€с€傞泴婵€绱犲彈浣?ER)鍜屽瓡閰?PR)琛ㄨ揪闃存€с€侟br/>鍦ㄦ湰搴撻€氳繃鏀師浣撴娴嬨€侟br/>鍦ㄦ湰搴撻€氳繃STR妫€娴嬨€侟/span> |
鍔ㄧ墿绉嶅埆
Organism |
浜裹/span> |
鎬у埆
Gender |
濂颤/span> |
缁勭粐鏉ユ簮
Tissue and Cell Type |
涔虫埧; 鍘熷彂鎬у绠＄檶; 3绾? IIB鏈烖/span> |
| 褰㈡€?br/>Morphology |
涓婄毊缁嗚優鏍饵/span> |
| 鍩瑰吇鍩哄拰娣诲姞鍓侟br/>Complete Growth Medium and Culture Conditions |
RPMI 1640鍩瑰吇鍩?GIBCO by life technologies ,璐у彿C11875500BT)锛?0%锛涗紭璐ㄨ儙鐗涜娓咃紝10%銆侟br/>姘旂浉锛氱┖姘旓紝95%锛涗簩姘у寲纰筹紝5%銆侟br/>娓╁害锛?7鎽勬皬搴︺€侟/span> |
渚涘簲闄愬埗
Permits and Restrictions |
A銆備粎渚涚爺绌朵箣鐢ㄣ€侟/span> |
| REFERENCE |
Tomlinson GE et al. Chaacterization of a breast cancer cell line derived from a germ-line BRCA1 mutation. Cancer Res. 58:3237-3242, 1998 PubMed: 98363228Gazdar AF et al. Charaterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998 PubMed: 99049517Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
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鐩稿叧浜у搧
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鍦ㄧ嚎鐣欒█
