产品介绍
| 目录叶br/>Serial |
SCSP-5064 |
| 标识?br/>Identifier |
CSTR:19375.09.3101HUMSCSP5064 |
描述
Description |
超微结构的特征包括一些微绒毛、少许细丝、一些小线粒体、发达的高尔基体和内质网、一些脂滴、多层体和次级溶酶体，无病毒颗粒。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误，STR鉴定结果为：Amelogenin: X：br/>CSF1PO: 10,11：br/>D13S317: 11,12：br/>D16S539: 12：br/>D5S818: 11,12：br/>D7S820: 8,12：br/>THO1: 6,8：br/>TPOX: 8,11：br/>vWA: 15,17 |
动物种别
Organism |
人；白人 |
性别
Gender |
男性，49?/span> |
组织来源
Tissue and Cell Type |
肾；来自转移灶：皮肤 |
| 形?br/>Morphology |
上皮样，贴壁生长 |
| 培养基和添加剁br/>Complete Growth Medium and Culture Conditions |
人肾透明细胞癌细胞Caki-1完全培养液配?100 ml)９br/>McCoy's 5A(Gibco, 16600082) 90 ml
FBS (Gibco) 10 ml
参考传代周期：每周2-3欠br/>参考传代比例：1:2-1:4
参考换液频率：每周2-3欠br/>冻存液配方：完全培养?5%，DMSO 5%
气相：空气，95%；二氧化碳，5% 温度?7摄氏度、/span> |
供应限制
Permits and Restrictions |
A。仅供研究之用、/span> |
| REFERENCE |
Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 |
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