半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-5027 |
标识符
Identifier |
CSTR:19375.09.3101HUMSCSP5027 |
描述
Description |
人结直肠腺癌细胞Caco-2 [Caco2]分离自直肠原位癌，适于转染。当细胞长满时，可表现出肠上皮细胞分化的特征。Caco-2细胞表达维甲酸结合蛋白I和维甲酸结合蛋白II。每个批次均通过本库支原体检测，结果为阴性。冻存时为P30代。经本库STR检测无误，STR鉴定结果为：Amelogenin: X,CSF1PO: 11,D13S317: 11,13,14,D16S539: 12,13,D5S818: 12,13,D7S820: 11,12,THO1: 6,TPOX: 9,11,vWA: 16,18 |
动物种别
Organism |
人；白人 |
性别
Gender |
男性，72岁 |
组织来源
Tissue and Cell Type |
结肠 |
形态
Morphology |
贴壁生长；细胞形态不均一，有部分含有液泡的巨大细胞 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
人结直肠腺癌细胞Caco-2完全培养液 配方(100 ml)：
MEM (Invitrogen，11090081) 77 ml
FBS (Gibco) 20 ml
Glutamax(invitrogen 35050061) 1 ml
Non-essential Amino Acids, 100X (Invitrogen, 11140050) 1 ml
Sodium Pyruvate 100 mM Solution(invitrogen 11360070) 1 ml
参考传代周期：4-6天
参考传代比例：1 : 3
冻存液：完全培养液95%，DMSO 5%
气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。
注意事项：
1.复苏或传代后细胞贴壁较慢，大量细胞会漂浮并存在一些碎片，最终会以岛状形态贴壁并扩散生长。为了避免干扰细胞贴壁，48h内不要换液；细胞形态不均一，有一些含有液泡的巨大细胞，该细胞培养过程中会有黑色颗粒物，属正常现象；消化时间5-10 min。
2.该细胞对血清质量较为敏感，我库建议您使用品牌优质胎牛血清进行培养或选择订购我库配套Caco-2完全培养液，货号为SCSP-671。
3.我库冻存时，每支冻存管约含1×106细胞量，体积为500μl，预期存活率70%，建议复苏至1个T25培养瓶中。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034Adachi A, et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. PubMed: 3640832Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874Cohen MB, et al. Receptors for Escherichia coli heat stable enterotoxin in human intestine and in a human intestinal cell line (Caco-2). J. Cell. Physiol. 156: 138-144, 1993. PubMed: 8100232Basson MD, et al. Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994. PubMed: 8077287Heinen CD, et al. Microsatellite instability in colorectal adenocarcinoma cell lines that have full-length adenomatous polyposis coli protein. Cancer Res. 55: 4797-4799, 1995. PubMed: 7585508Gilbert T, Rodriguez-Boulan E. Induction of vacuolar apical compartments in the Caco-2 intestinal epithelial cell line. J. Cell Sci. 100: 451-458, 1991. PubMed: 1808199Rigothier MC, et al. A new in vitro model of Entamoeba histolytica adhesion, using the human colon carcinoma cell line Caco-2: scanning electron microscopic study. Infect. Immun. 59: 4142-4146, 1991. PubMed: 1937772Levin MS. Cellular retinol-binding proteins are determinants of retinol uptake and metabolism in stably transfected Caco-2 cells. J. Biol. Chem. 268: 8267-8276, 1993. PubMed: 8463337Trotter PJ, Storch J. Fatty acid esterification during differentiation of the human intestinal cell line Caco-2. J. Biol. Chem. 268: 10017-10023, 1993. PubMed: 8387510Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293Grindstaff KK, et al. Translational regulation of Na,K-ATPase alpha1 and beta1 polypeptide expression in epithelial cells. J. Biol. Chem. 271: 23211-23221, 1996. PubMed: 8798517 |
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