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细胞培养基 >> 细胞株
Caco-2 [Caco2]人结直肠腺癌细胞(2017年新引进)
Caco-2 [Caco2]人结直肠腺癌细胞(2017年新引进)图片
半岛bd体育手机客户端 介绍
目录号
Serial
SCSP-5027
标识符
Identifier
CSTR:19375.09.3101HUMSCSP5027
描述
Description
人结直肠腺癌细胞Caco-2 [Caco2]分离自直肠原位癌,适于转染。当细胞长满时,可表现出肠上皮细胞分化的特征。Caco-2细胞表达维甲酸结合蛋白I和维甲酸结合蛋白II。每个批次均通过本库支原体检测,结果为阴性。冻存时为P30代。经本库STR检测无误,STR鉴定结果为:Amelogenin: X,CSF1PO: 11,D13S317: 11,13,14,D16S539: 12,13,D5S818: 12,13,D7S820: 11,12,THO1: 6,TPOX: 9,11,vWA: 16,18
动物种别
Organism
人;白人
性别
Gender
男性,72岁
组织来源
Tissue and Cell Type
结肠
形态
Morphology
贴壁生长;细胞形态不均一,有部分含有液泡的巨大细胞
培养基和添加剂
Complete Growth Medium and Culture Conditions
人结直肠腺癌细胞Caco-2完全培养液 配方(100 ml):
MEM (Invitrogen,11090081) 77 ml
FBS (Gibco) 20 ml
Glutamax(invitrogen 35050061) 1 ml
Non-essential Amino Acids, 100X (Invitrogen, 11140050) 1 ml
Sodium Pyruvate 100 mM Solution(invitrogen 11360070) 1 ml
参考传代周期:4-6天
参考传代比例:1 : 3
冻存液:完全培养液95%,DMSO 5%
气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。
注意事项:
1.复苏或传代后细胞贴壁较慢,大量细胞会漂浮并存在一些碎片,最终会以岛状形态贴壁并扩散生长。为了避免干扰细胞贴壁,48h内不要换液;细胞形态不均一,有一些含有液泡的巨大细胞,该细胞培养过程中会有黑色颗粒物,属正常现象;消化时间5-10 min。
2.该细胞对血清质量较为敏感,我库建议您使用品牌优质胎牛血清进行培养或选择订购我库配套Caco-2完全培养液,货号为SCSP-671。
3.我库冻存时,每支冻存管约含1×106细胞量,体积为500μl,预期存活率70%,建议复苏至1个T25培养瓶中。
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCE Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034Adachi A, et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. PubMed: 3640832Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874Cohen MB, et al. Receptors for Escherichia coli heat stable enterotoxin in human intestine and in a human intestinal cell line (Caco-2). J. Cell. Physiol. 156: 138-144, 1993. PubMed: 8100232Basson MD, et al. Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994. PubMed: 8077287Heinen CD, et al. Microsatellite instability in colorectal adenocarcinoma cell lines that have full-length adenomatous polyposis coli protein. Cancer Res. 55: 4797-4799, 1995. PubMed: 7585508Gilbert T, Rodriguez-Boulan E. Induction of vacuolar apical compartments in the Caco-2 intestinal epithelial cell line. J. Cell Sci. 100: 451-458, 1991. PubMed: 1808199Rigothier MC, et al. A new in vitro model of Entamoeba histolytica adhesion, using the human colon carcinoma cell line Caco-2: scanning electron microscopic study. Infect. Immun. 59: 4142-4146, 1991. PubMed: 1937772Levin MS. Cellular retinol-binding proteins are determinants of retinol uptake and metabolism in stably transfected Caco-2 cells. J. Biol. Chem. 268: 8267-8276, 1993. PubMed: 8463337Trotter PJ, Storch J. Fatty acid esterification during differentiation of the human intestinal cell line Caco-2. J. Biol. Chem. 268: 10017-10023, 1993. PubMed: 8387510Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293Grindstaff KK, et al. Translational regulation of Na,K-ATPase alpha1 and beta1 polypeptide expression in epithelial cells. J. Biol. Chem. 271: 23211-23221, 1996. PubMed: 8798517
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