半岛bd体育手机客户端 介绍
目录号
Serial |
TCHu 146 |
标识符
Identifier |
CSTR:19375.09.3101HUMTCHu146 |
描述
Description |
这株细胞分离自直肠原位癌。 当长到满时，细胞表现出特征性的肠上皮细胞分化。 Caco-2细胞表达维甲酸结合蛋白I和维甲酸结合蛋白II，并呈角质蛋白阳性。
在本库通过支原体检测。
在本库通过STR检测。 |
动物种别
Organism |
人 |
性别
Gender |
男 |
组织来源
Tissue and Cell Type |
结肠,结直肠腺癌 |
形态
Morphology |
上皮细胞 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，80%；优质胎牛血清，20%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Didier, E.S., et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDSpatients. J. Eukaryot. Microbiol 43: 34-43. 1996 PubMed:96151459Jumade, C., and Malo, C. Caco-2 cells cultured in serum-free medium as a model for the study of enterocytic differentiation in vitro. J. Cell. Physiol. 149: 24-33, 1991 PubMed: 92042315Fogh, J., et al. Absence of HeLa ceil contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977 PubMed: 77097006Fogh, J., et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977 PubMed:77210034Adachi, A., et al. Productive, persistent infection of human co]orectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987 PubMed: 87061242Trainer, D.L., et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296. 1988 PubMed:88114170Cohen, M.B., et al. Receptors for Escherichia coil heat stable enterotoxin in human intestine and in a human intestinal cell line (Caco-2). J. Cell, Physiol. 156: 138-144,1993 PubMed: 93390921Basson, M.D., et al. Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994 PubMed: 94357990Heinen, C.D., et al. Microsatellite instability in colorectal adenocareinoma cell lines that have full-length adenomatous polyposis coil protein. Cancer Res. 55: 4797-4799, 1995 PubMed: 96046575GilberL, T., Rodriguez-Boulan, E. Induction of vacuolar apical compartments in the Caco-2 intestinal epithelial cell line. J. Cell Sci. 100: 451-458, 1991 PubMed: 92226167Rigothier, M.C., et al. A new in vitro model of Entamoeba histolytica adhesion, using the human colon carcinoma cell line Caco-2: scanning electron microscopic study. Infect. Immun. 59: 4142-4146, 1991 PubMed: 92040089Levin, M.S. Cellular retinol-binding proteins are determinants of retinol uptake and metabolism in stably transfected Caco-2 cells. J. Biol. Chem. 268: 8267-8276.1993 PubMed: 93216811Trotter, P.J., and Storch, J. Fatty acid esterification during differentiation of the human intestinal cell line Caco-2. J. Biol. Chem. 268: 10017-10023, 1993 PubMed: 93252877Santoro, I.M., Groden, J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997 PubMed:97164672White, L.J., et al. Attachment and entry of recombinant norwalk virus eapsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996 PubMed: 96386543Baier, L.J., et al. A polymorphism in the human intestinal fatty acid binding protein alters fatty acid transport across Caco-2 cells. J. Biol. Chem. 271: 10892-19896. 1996 PubMed: 96210027Kutchera, W., et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93:4816-4820, 1996 PubMed: 96209815Grindstaff, K.K. et al. Translational regulation of Na,K-ATPase alpha1 and beta1 polypeptide expression in epithelial cells. J. Biol. Chem. 271: 23211-23221, 1996 PubMed: 96394411Hay, R. J., Caputo, J. L., and Macy, M. L. Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
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