细胞培养基 >> 细胞株
A-375 [A375]人恶性黑色素瘤细胞(2013年新引进)
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介绍 目录号 Serial | SCSP-533 | 标识符 Identifier | CSTR:19375.09.3101HUMSCSP533 | 描述 Description | 人恶性黑色素瘤细胞A-375 [A375]来源于一名54岁的女性。在裸鼠中有致瘤性。每个批次均通过本库支原体检测,结果为阴性。经本库STR检测结果无误。STR结果如下:D5S818: 12;D13S317: 11,14;D7S820:9;D16S539: 9;vWA: 16,17;THO1: 8;Amelogenin: X;TPOX: 8,10;CSF1PO: 11,12 | 动物种别 Organism | 人 | 性别 Gender | 女性,54岁 | 组织来源 Tissue and Cell Type | 皮肤 | 形态 Morphology | 上皮样,贴壁生长 | 培养基和添加剂 Complete Growth Medium and Culture Conditions | 人皮肤黑色素瘤细胞A-375[A375]细胞完全培养液配方(100 ml): DMEM (Invitrogen, 11960044)88 ml FBS (Gibco) 10 ml Glutamax (Invitrogen, 35050061) 1 ml Sodium Pyruvate (Invitrogen, 11360-070) 1 ml(终浓度1 mM) 参考传代周期:2-3天 参考传代比例:1:3-1:5 参考换液频率:2-3天 冻存液:完全培养液95%,DMSO 5% 细胞状态:贴壁生长 气相:空气,95%;二氧化碳,5%。温度:37摄氏度。 | 供应限制 Permits and Restrictions | A。仅供研究之用。 | REFERENCE | Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033 Cormier JN, et al. Heterogeneous expression of melanoma-associated antigens and HLA-A2 in metastatic melanoma in vivo. Int. J. Cancer 75: 517-524, 1998. PubMed: 9466650 Sharma SD, et al. Melanotropic peptide-conjugated beads for microscopic visualization and characterization of melanoma melanotropin receptors. Proc. Natl. Acad. Sci. USA 93: 13715-13720, 1996. PubMed: 8943000 |
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