半岛bd体育手机客户端 介绍
目录号
Serial |
GNHu17 |
标识符
Identifier |
CSTR:19375.09.3101HUMGNHu17 |
描述
Description |
293细胞株插入SV40 T-抗原的温度敏感基因后产生的高转染效率的衍生株称为293T。
在本库通过支原体检测。
在本库通过STR检测。 |
动物种别
Organism |
人 |
性别
Gender |
*** |
组织来源
Tissue and Cell Type |
肾 |
形态
Morphology |
上皮细胞 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Sena-Esteves M , et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999. PubMed: 10559361Pensiero M , et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999Pensiero M , et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 6,329,199 dated Dec 11 2001Pear WS , et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960 |
|
|
相关半岛bd体育手机客户端
|
